You searched for: Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
Organelle-ID™ RGB reagent II
Supplier: ENZO LIFE SCIENCES
Mixture of fluorescent dyes for detection of lysosomes, endoplasmic reticulum and nucleic acids
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CF™ dye streptavidin and monoclonal mouse anti-biotin conjugates
Supplier: Biotium
CF™ dyes are superior to other fluorescent dyes for protein labelling due to advantages in brightness, photostability and water solubility.
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Tyramide amplification buffer plus
Supplier: Biotium
An optimized buffer with enhanced sensitivity for tyramide signal amplification of immunostaining in cells or tissue sections.
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Antibody labelling kits, Mix-n-Stain™ FITC
Supplier: Biotium
Mix-n-Stain™ FITC antibody labelling kits utilise a technology that allows rapid antibody labelling with green fluorescent dye (FITC) without a purification step.
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HRP-conjugate stabiliser, SeramunStab® STA2
Supplier: Apollo Scientific
Liquid product red odourless pH-value 7,3 to 7,5 Boiling point 102 °C. Stabiliser for a broad range of HRP conjugates.
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ExoBrite™ True EV Membrane Stains
Supplier: Biotium
Lipophilic membrane dyes designed specifically for EV research and the best choice for general pan-EV staining.
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Wheat germ agglutinin (WGA) conjugates
Supplier: Biotium
A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.
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Fluorescein-12-ddUTP
Supplier: ENZO LIFE SCIENCES
Fluorescein-12-ddUTP can be used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. It also serves as a substrate for E.coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase. Oligonucleotide probes labelled with fluorescein-12-ddUTP can be detected by direct fluorescence detection (green fluorescence) or by methods using horseradish peroxidase- or alkaline phosphatase-linked anti-fluorescein antibodies.
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Bio-16-ddUTP, tetralithium salt
Supplier: ENZO LIFE SCIENCES
Bio-16-ddUTP (Biotin-16-2’,3’-dideoxyuridine-5’-triphosphate) can be used for 3’-end labelling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-16-ddUMP. Biotin-labelled oligonucleotides produced by this reaction are suitable as hybridisation probes and nucleic acid array assays. It is also a substrate for Taq DNA polymerase and other DNA polymerases.
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Gold 550 dUTP
Supplier: ENZO LIFE SCIENCES
Gold 550 [Cyanine-3E] dUTP is a cyanine-3 derivative with enhanced spectral properties. This nucleotide analogue can replace TTP in reactions in which it serves as a substrate for E.coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.
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Orange 552 dUTP (lyophilised)
Supplier: ENZO LIFE SCIENCES
Orange 552 dUTP (lyophilised)
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CDI amine reactive resin
Supplier: G-Biosciences
CDI amine reactive agarose consists of 6% cross-linked agarose activated with CDI (1,1’-carbonyl diimidazole) to form reactive imidazole carbamates.
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CF™ Dye lectin conjugates
Supplier: Biotium
A lectin labelled with our superior fluorescent CF® dyes is an effective and widely used cell surface stain for yeast, fungi, and mammalian cells.
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Annexin V Near IR CF® dye conjugates
Supplier: Biotium
Near-IR Annexin V fluorescent conjugates can be used to detect apoptotic cells and is suitable for in vivo imaging.
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Dye tyramide, CF®
Supplier: Biotium
Fluorescent CF® dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolour immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).
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Red 580 dUTP (lyophilised)
Supplier: ENZO LIFE SCIENCES
Red 580 dUTP (lyophilised)
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D-luciferin luminescent substrates
Supplier: Biotium
Luciferin is a substrate for the enzyme firefly luciferase.
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Nanobody labelling kits, Mix-n-Stain™
Supplier: Biotium
The simplicity and convenience of Mix-n-Stain™ labelling of nanobodies with fluorescent CF® dyes or biotin.
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Biotin picolyl azide
Supplier: Biotium
Biotin picolyl azide reacts with alkyne to form 1,2,3-triazole by 1,3-dipolar Huisgen cycloaddition through the use of a much lower copper (I) concentration without sacrificing reaction efficiency.
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ORGANELLE-ID-RGB® III Assay kit
Supplier: ENZO LIFE SCIENCES
TheORGANELLE-ID-RGB® III assay kit contains Golgi apparatus-selective, endoplasmic reticulum-selective and nucleus-selective dyes suitable for live cell staining.
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100-reaction single-round RNA amplification and Biotin labeling system
Supplier: ENZO LIFE SCIENCES
Improved data quality and analysis results through greater biotin incorporation.
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Photoreactive dyes and reagents
Supplier: Biotium
INA (5-iodonaphthyl-1-azide) is a lipophilic photoreactive probe, which has been used to selectively label membrane-embedded cysteine residues of proteins.
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Peroxidase anti-peroxidase (PAP) soluble complexes
Supplier: Rockland Immunochemicals
Peroxidase anti-peroxidase soluble complexes are used for peroxidase enzymatic labelling experiments.
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IgG Biotinylation kits, HOOK™
Supplier: G-Biosciences
HOOK™ IgG biotinylation kits are designed for the biotinylation of IgG molecules by immobilising the IgG molecules on a solid support. Nickel chelating resin allows for the rapid removal of uncoupled biotin and therefore eliminates the need for further dialysis or desalting of the biotinylated antibody.
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Reactive dye conjugates
Supplier: Biotium
Reactive dyes containing an amine functional group.
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BIOARRAY™ Low Input RNA Amplification with Biotin Labeling System
Supplier: ENZO LIFE SCIENCES
The BIOARRAY™ Low Input RNA Amplification with Biotin Labeling System provides an optimised protocol and reagents for the production of biotin-labeled antisense RNA (aRNA) from total cellular RNA samples in less than 24 hours for array analysis.
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Stabiliser for proteins and standards, SeramunStab® Stpur
Supplier: Apollo Scientific
Liquid product colourless to slightly yellow or greenish odourless pH 7,3 to 7,5 boiling point 104 °C. Stabiliser for controls and protein standards.
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Maackia amurensis Lectin (MAA/MAL II) (FITC)
Supplier: US Biological
Maackia amurensis Lectin (MAA/MAL II) is labelled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin.
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Small ligand labelling kits, Mix-n-Stain™ CF® Dye
Supplier: Biotium
Easily label SNAP-tag®, CLIP-tag™, HaloTag®, or other ligands with fluorescent dyes, no purification needed. Cell membrane-permeant and impermeant dye options for intracellular or surface targets.
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HRP - conjugate stabiliser, SeramunStab® STM
Supplier: Apollo Scientific
Liquid product orange to red odourless pH 6,4 to 6,6. Stabiliser for conjugates with monomeric HRP. Preservation of very low conjugate concentrations.