You searched for: Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
AMC (7-Amino-4-methylcoumarin) standard
Supplier: ENZO LIFE SCIENCES
Fluorogenic chromophore. Ex(max): 380nm, Em(max): 460nm. Reference compound.
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femtoCHROMO™- HRP Substrate for chromogenic detection
Supplier: G-Biosciences
A ready to use modified TMB (3, 3’, 5, 5’-Tetramethylbenzidine) substrate is used that generates a dark blue precipitate in the presence of horseradish peroxidase.
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D-luciferin luminescent substrates
Supplier: Biotium
Luciferin is a substrate for the enzyme firefly luciferase.
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CGH labeling kit, CYTAG®
Supplier: ENZO LIFE SCIENCES
Comparative genomic hybridisation (CGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. CGH provides an understanding of genetic disorders, cancers and other genomic aberrations.
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Antibody labelling kits, Mix-n-Stain™ CF™ Dye
Supplier: Biotium
Label your antibody with one of our superior CF® Dyes in 30 minutes without a purification step. Labelling tolerates many common buffer components including BSA and ascites.
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Red 580 dUTP (lyophilised)
Supplier: ENZO LIFE SCIENCES
Red 580 dUTP (lyophilised)
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Nuclear-ID™ Blue/Red cell viability reagent (GFP-Certified™)
Supplier: ENZO LIFE SCIENCES
Cell viability dye
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Nanobody labelling kits, Mix-n-Stain™
Supplier: Biotium
The simplicity and convenience of Mix-n-Stain™ labelling of nanobodies with fluorescent CF® dyes or biotin.
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Reactive quencher dyes
Supplier: Biotium
Reactive quenchers that act as acceptors in fluorescence resonance energy transfer (FRET) applications.
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Organelle-ID™ RGB reagent II
Supplier: ENZO LIFE SCIENCES
Mixture of fluorescent dyes for detection of lysosomes, endoplasmic reticulum and nucleic acids
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Tyramide amplification buffer plus
Supplier: Biotium
An optimized buffer with enhanced sensitivity for tyramide signal amplification of immunostaining in cells or tissue sections.
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HRP - conjugate stabiliser, SeramunStab® STC
Supplier: Apollo Scientific
Liquid product red odourless pH-value 7,2 to 7,4 boiling point 103 °C. Optimised for background reduction.
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CF® Dye dCTP conjugates
Supplier: Biotium
CF® dye-dCTP can be used to synthesise probes for in situ hybridisation, microarray, or to synthesise labelled DNA probes for in situ hybridisation and nucleic acid blotting applications.
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Carbohydrate coupling resin
Supplier: G-Biosciences
Carbohydrate coupling resin is designed for the covalent immobilisation of carbohydrate containing molecules, including glycoproteins, to agarose beads. It is ideal for the coupling of polyclonal antibodies as it allows for the optimal orientation of the antibodies for affinity purification.
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CF® Dye BCN
Supplier: Biotium
CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labelling reactions are also available.
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CF™ dye streptavidin and monoclonal mouse anti-biotin conjugates
Supplier: Biotium
CF™ dyes are superior to other fluorescent dyes for protein labelling due to advantages in brightness, photostability and water solubility.
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NEDDylation kit
Supplier: ENZO LIFE SCIENCES
This kit provides the means of generating a thioester-linked NEDD8-conjugated E2 enzyme, utilizing the first two steps in the NEDD8 cascade, for use in the NEDDylation of E3 ligases and target substrate proteins (user supplied). A NEDD8-specific antibody is provided for detection of NEDDylated proteins via SDS-PAGE and Western blotting.
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Mix-n-Stain™ Cyanine dye antibody labelling kits
Supplier: Biotium
Revolutionary technology allows antibody labelling with Cyanine 555 (Cy®3) or Cyanine 647 (Cy®5) in 30 minutes without a purification step. The labelling procedure tolerates many common buffer components and antibody stabilisers.
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Tyramide amplification kit with HRP streptavidin and CF® dye or biotin tyramide
Supplier: Biotium
Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.
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Stabiliser for sensitive biomolecules, SeramunStab® STB
Supplier: Apollo Scientific
Liquid product colourless to slightly yellow, freshly solutions opaleszent, odour weakly pH 7,3 to 7,5 boiling point 104 °C. Stabiliser for sensitive biomolecules like proteohormones or membrane proteins.
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Organelle-ID™ RGB reagent I
Supplier: ENZO LIFE SCIENCES
Mixture of fluorescent dyes for detection of lysosomes, mitochondria and nucleic acids
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100-reaction single-round RNA amplification and Biotin labeling system
Supplier: ENZO LIFE SCIENCES
Improved data quality and analysis results through greater biotin incorporation.
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Biotin kits, Micro HOOK™
Supplier: G-Biosciences
The micro HOOK™ Biotin kits are designed to label small amounts of proteins, with each kit designed for 8 to 10 labellings of 50 to 250 µg protein/reaction.
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Nick Translation DNA Labelling System 2.0
Supplier: ENZO LIFE SCIENCES
The Nick Translation DNA labelling system 2.0 provides a simple and efficient method for generating labelled DNA. The complete system includes all of the necessary reagents required for 50 Nick translation reactions and is recommended for labeling of double stranded DNA larger than 1 kb for fluorescent in situ hybridisation (FISH) applications.
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Photoreactive dyes and reagents
Supplier: Biotium
INA (5-iodonaphthyl-1-azide) is a lipophilic photoreactive probe, which has been used to selectively label membrane-embedded cysteine residues of proteins.
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Biotin reagents, OneQuant™ HOOK™
Supplier: G-Biosciences
The OneQuant™ format prevents loss of reagent due to repeated weighing as each vial contains only 1 - 2 mg HOOK™ Biotin Reagent.
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Carboxyl coupling resin
Supplier: G-Biosciences
Carboxyl coupling resin consists of 6% cross-linked agarose with covalently linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. Molecules, including proteins, peptides and nucleic acids, are covalently coupled to the free primary amines, and the stable columns are ideal for affinity purification of antibodies and other interacting partners.
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Peroxidase anti-peroxidase (PAP) soluble complexes
Supplier: Rockland Immunochemicals
Peroxidase anti-peroxidase soluble complexes are used for peroxidase enzymatic labelling experiments.
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Bio-N⁶-ddATP
Supplier: ENZO LIFE SCIENCES
Bio-N⁶-ddATP (Biotin-N⁶-(2’,3’-dideoxy)adenosine-5’ triphosphate) can be used for 3’-end labelling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-N⁶-ddAMP. It is also a substrate for Taq DNA Polymerase and other DNA polymerases.
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Antibody labelling kits, Mix-n-Stain™ FITC
Supplier: Biotium
Mix-n-Stain™ FITC antibody labelling kits utilise a technology that allows rapid antibody labelling with green fluorescent dye (FITC) without a purification step.