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Protein Linking and Labeling Systems

Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.

Antibody labelling kits, Mix-n-Stain™ CF™ Dye

Antibody labelling kits, Mix-n-Stain™ CF™ Dye

Supplier: Biotium

Label your antibody with one of our superior CF® Dyes in 30 minutes without a purification step. Labelling tolerates many common buffer components including BSA and ascites.

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Biotin conjugation estimation, HOOK™ BiotinQuant

Supplier: G-Biosciences

HOOK™ BiotinQuant is designed for the estimation of biotin conjugation. It measures biotin using HABA [4’-hydroxyazobenzene-2-carboxylic acid] dye. HABA binds with avidin at the biotin-binding site.

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R-Phycoerythrin (RPE)

R-Phycoerythrin (RPE)

Supplier: Columbia Biosciences

Many applications and instruments were developed specifically for R-phycoerythrin.

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CF™ Dyes Cholera Toxin Subunit B, CF™ Dye Conjugates

CF™ Dyes Cholera Toxin Subunit B, CF™ Dye Conjugates

Supplier: Biotium

Cholera toxin subunit B conjugated to Biotium's next-generation fluorescent CF™ dyes.

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HRP - conjugate stabiliser, SeramunStab® STC

Supplier: Apollo Scientific

Liquid product red odourless pH-value 7,2 to 7,4 boiling point 103 °C. Optimised for background reduction.

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CF® Dye dCTP conjugates

Supplier: Biotium

CF® dye-dCTP can be used to synthesise probes for in situ hybridisation, microarray, or to synthesise labelled DNA probes for in situ hybridisation and nucleic acid blotting applications.

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Coelenterazine Substrates for Renilla Luciferase and Apoaequorin

Supplier: Biotium

Coelenterazine and its analogs are luminescent enzyme substrates for apoaequorin and Renilla luciferase.

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100-reaction single-round RNA amplification and Biotin labeling system

Supplier: ENZO LIFE SCIENCES

Improved data quality and analysis results through greater biotin incorporation.

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Organelle-ID™ RGB reagent I

Supplier: ENZO LIFE SCIENCES

Mixture of fluorescent dyes for detection of lysosomes, mitochondria and nucleic acids

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Photoreactive dyes and reagents

Supplier: Biotium

INA (5-iodonaphthyl-1-azide) is a lipophilic photoreactive probe, which has been used to selectively label membrane-embedded cysteine residues of proteins.

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CF® Dye hydrazide

CF® Dye hydrazide

Supplier: Biotium

Fluorescent CF® Dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.

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Mix-n-Stain™ Cyanine dye antibody labelling kits

Mix-n-Stain™ Cyanine dye antibody labelling kits

Supplier: Biotium

Revolutionary technology allows antibody labelling with Cyanine 555 (Cy®3) or Cyanine 647 (Cy®5) in 30 minutes without a purification step. The labelling procedure tolerates many common buffer components and antibody stabilisers.

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Biotin kits, Micro HOOK™

Supplier: G-Biosciences

The micro HOOK™ Biotin kits are designed to label small amounts of proteins, with each kit designed for 8 to 10 labellings of 50 to 250 µg protein/reaction.

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Stabiliser for calibrators and controls, SeramunStab® STD

Supplier: Apollo Scientific

Liquid product yellow odourless pH-value 7,3 to 7,5 Boiling point 102 °C. Stabiliser for complex mixtures of biomolecules.

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Nick Translation DNA Labelling System 2.0

Nick Translation DNA Labelling System 2.0

Supplier: ENZO LIFE SCIENCES

The Nick Translation DNA labelling system 2.0 provides a simple and efficient method for generating labelled DNA. The complete system includes all of the necessary reagents required for 50 Nick translation reactions and is recommended for labeling of double stranded DNA larger than 1 kb for fluorescent in situ hybridisation (FISH) applications.

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Peroxidase anti-peroxidase (PAP) soluble complexes

Supplier: Rockland Immunochemicals

Peroxidase anti-peroxidase soluble complexes are used for peroxidase enzymatic labelling experiments.

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Biotin reagents, OneQuant™ HOOK™

Supplier: G-Biosciences

The OneQuant™ format prevents loss of reagent due to repeated weighing as each vial contains only 1 - 2 mg HOOK™ Biotin Reagent.

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Carbohydrate coupling resin

Carbohydrate coupling resin

Supplier: G-Biosciences

Carbohydrate coupling resin is designed for the covalent immobilisation of carbohydrate containing molecules, including glycoproteins, to agarose beads. It is ideal for the coupling of polyclonal antibodies as it allows for the optimal orientation of the antibodies for affinity purification.

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CGH labeling kit, CYTAG®

Supplier: ENZO LIFE SCIENCES

Comparative genomic hybridisation (CGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. CGH provides an understanding of genetic disorders, cancers and other genomic aberrations.

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B-Phycoerythrin (BPE)

B-Phycoerythrin (BPE)

Supplier: Columbia Biosciences

Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore.

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CF® Dye BCN

CF® Dye BCN

Supplier: Biotium

CF® Dyes with a BCN group react with azides via a copper-free bioorthogonal reaction to label target molecules for surface staining of live cells or cell extracts. Membrane-permeant CF® Dye BCN conjugates for intracellular labelling reactions are also available.

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Dye conjugates dUTP CF®

Supplier: Biotium

CF® dye-dUTP can be used for TUNEL assay, microarray, or to synthesize labelled DNA probes for in situ hybridisation and nucleic acid blotting applications.

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Orange 552 dUTP (lyophilised)

Orange 552 dUTP (lyophilised)

Supplier: ENZO LIFE SCIENCES

Orange 552 dUTP (lyophilised)

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Tyramide amplification kit with HRP streptavidin and CF® dye or biotin tyramide

Supplier: Biotium

Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.

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Organelle-ID™ RGB reagent II

Supplier: ENZO LIFE SCIENCES

Mixture of fluorescent dyes for detection of lysosomes, endoplasmic reticulum and nucleic acids

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Maxi fluorescent antibody labelling kits, Mix-n-Stain™, 1 mg labelling

Supplier: Biotium

The simplicity and convenience of Mix-n-Stain™ labelling, for conjugation of 1 mg IgG with fluorescent CF® dyes or other dyes.

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Tyramide amplification buffer plus

Tyramide amplification buffer plus

Supplier: Biotium

An optimized buffer with enhanced sensitivity for tyramide signal amplification of immunostaining in cells or tissue sections.

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CF™ dye streptavidin and monoclonal mouse anti-biotin conjugates

Supplier: Biotium

CF™ dyes are superior to other fluorescent dyes for protein labelling due to advantages in brightness, photostability and water solubility.

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Reactive dye conjugates

Supplier: Biotium

Reactive dyes containing an amine functional group.

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Biotin SE protein labelling kit

Supplier: Biotium

This kit contains everything needed to label antibodies or other proteins with biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labelling reactions of up to 1 mg protein per reaction.

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