Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Sigma-Aldrich®

Supplier: Merck

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble, and protected from degradation. This concentrate can be easily diluted to 1X or 0,5X before use (with molecular biology grade water).

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Glucose hemolysing solution

Supplier: DIAGNOSTIC SYSTEMS DIASYS

For use with glucose Gluc-DH FS and glucose Gluc-DH FSC.

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Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Sigma-Aldrich®

Supplier: Merck

Electrophoresis, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)

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Tris pH Buffer Solution

Supplier: CHEMPUR

rr tris-HCl buffer, HCl, pH value: 8.5, Clear

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Buffer, TRIS solution pH 9,0 (1 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 9,0 (1 mol/l)

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TISAB II Solution, Supelco®

Supplier: Merck

TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.

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Buffer solutions in sachets, perpHect® Pouches, Orion™

Supplier: Thermo Orion

Solutions in sachets ensure optimum precision. The sachet provides a perfect liquid seal and good protection from light.

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Triethylammonium bicarbonate buffers, for ion-exchange chromatography, Sigma-Aldrich®

Supplier: Merck

Buffer for use in ion-exchange chromatography and electrophoresis.

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Buffer, HEPES solution (1 mol/L), Sigma-Aldrich®

Supplier: Merck

HEPES buffer solution (1 mol/l)

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Phosphate buffered saline (PBS), Sigma-Aldrich®

Supplier: Merck

Contents of one pouch, when dissolved in one liter of distilled or deionised water, will yield 0,01 M phosphate buffered saline (NaCl 0,138 M; KCl - 0,0027 M); TWEEN® 20 - 0,05%, pH 7,4, at 25 °C.

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Buffer, imidazole buffer solution (1 mol/L) BioUltra, Sigma-Aldrich®

Supplier: Merck

Imidazole buffer solution (1 mol/l)

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Buffer tablets, Lovibond®

Supplier: Lovibond

Ideal for preparing buffer solutions of 4, 7 and 10 pH units.

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Buffer, TRIS solution pH 7,2 (1 mol/L), Sigma-Aldrich®

Supplier: Merck

Cloning Buffer, TRIS buffer solution pH 7,2 (1 mol/l)

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Glycine buffer, Sigma-Aldrich®

Supplier: Merck

For use in the enzymatic determination of lactate using lactic dehydrogenase.

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Buffer, SSC solution 20X concentrate (saline-sodium citrate)

Supplier: SERACARE LIFE SCIENCES

Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)

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Buffer solutions

Supplier: Hanna

Solutions in sachets ensure optimum precision. The plastic-coated sachet provides a perfect liquid seal and good protection from light. The solutions should only be used once to avoid any danger of contamination. They also satisfy the requirements of the GLP and ISO standards. They have been adjusted using a pH meter that has been calibrated according to the NIST standards.