Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

PIPES-buffered saline (5X), pH 6,5

Supplier: Thermo Fisher Scientific

PIPES-buffered saline, 5X, pH value: 6,5, Colourless, Liquid

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Phosphate, 0,2 M buffer solution, pH 8,0

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,2 M, pH value: 8,0, Liquid

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Phosphate, 0,2 M buffer solution, pH 4,4

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,2 M, pH value: 4,4, Liquid

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IEF Cathode buffer (10X) with lysine and arginine

Supplier: Thermo Fisher Scientific

Running Buffer, IEF Cathode buffer (10X) with lysine and arginine

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Triethylammonium acetate buffer, BioUltra, volatile buffer, Fluka®

Supplier: Honeywell Chemicals

Triethylammonium acetate buffer, 1,0 M in H₂O, pH value: 7,0

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Laemmli SDS sample buffer, non-reducing (4X)

Supplier: Thermo Fisher Scientific

Loading Buffer, Laemmli SDS Sample Buffer, non-reducing, 4X

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10X Phosphate Buffered Saline (PBS), Sterile, Dulbecco's Formula, without Magnesium and Calcium

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)

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Buffer Capsules

Supplier: Greisinger

Cost-effective alternative: Buffer capsules and working sets. With buffer capsules, you can produce a 100 ml buffer solution in the most cost-effective manner. A particular advantage of the capsules is that their storage life is several years longer than that of ready-to-use solutions.

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GST buffer kit

Supplier: Cytiva

GST Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of GST-tagged proteins according to recommended protocols.

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Tris-Glycine (TG) buffer,, MP Biomedicals

Supplier: MP Biomedicals

Tris-glycine buffer is used for western blotting and gel electrophoresis.

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ISE sample conditioning buffers and solutions

Supplier: WTW

Ion strength adjustment solutions have multiple tasks: TISAB for fluoride measurements does not only adjust the pH value, by also decomplexes the fluoride-containing complex compounds. Other ion strength adjustment solutions ensure an adapted pH value as well as the addition of a suitable conducting salt to correctly capture the concentration.

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pH electrode calibration and maintenance solutions kit

Supplier: WTW

Adapted to different electrode types for calibration and maintenance.

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EDTA disodium salt solution, 0,2% in phosphate buffered saline, MP Biomedicals

Supplier: MP Biomedicals

EDTA disodium salt, (2%) solution, in phosphate buffered saline without calcium and magnesium. Used as a gentle non-enzymatic cell dissociation reagent.

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Low pH Phosphate SDS Sample Buffer

Supplier: EKSIGENT

SCIEX Low pH SDS phosphate sample buffer, 140 ml (40 mM Phosphate, pH 6,5, 1% SDS) allows you to use SCIEX’s Gold Standard CE-SDS with a China Pharmacopeia compliant buffer.

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Buffer solutions

Supplier: SI Analytics

Buffer solutions intended for extremely precise calibration.

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Protein A/G HP SpinTrap™ Buffer Kit

Supplier: Cytiva

Protein A/G HP SpinTrap™ Buffer kit is optimised for standard affinity-based enrichment/immunoprecipitation according to the protocol provided with protein A HP SpinTrap™, protein G HP SpinTrap™, and Ab SpinTrap™ columns.