Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Buffer, TE solution (TRIS-EDTA buffer) pH 7,4
Supplier: LONZA
Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 7,4
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Tween 20 blocking buffer, 1% in PBS (10X)
Supplier: Thermo Fisher Scientific
Blocking Buffer, Tween 20 Blocking Buffer, 1% in PBS (10X)
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HEPES protein extraction buffer (5X)
Supplier: Thermo Fisher Scientific
Thermo Fisher Scientific
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PIPES lysis buffer with NP-40 (2X)
Supplier: Thermo Fisher Scientific
PIPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific
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10X TAE buffer, liquid, Corning®
Supplier: Corning
Tris-Acetate-EDTA (TAE) buffer is used in DNA agarose gel electrophoresis, both in the agarose gel itself and the running buffer
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EDTA, 0.5M pH 8.0, Corning®
Supplier: Corning
EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+. These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity. The use of EDTA therefore allows for greater control over many experiments.
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10X Tris buffered saline, liquid, Corning®
Supplier: Corning
Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range.
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Tris-Acetate-EDTA (TAE) buffer, 50X
Supplier: PanReac AppliChem
50X concentrated solution of 2 M Tris, 1 M glacial acetic acid and 0,5 M EDTA.Na₂, pH 8,5 ±0,2.
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G-Biosciences Tris Buffered Saline with Tween 20 (10X)
Supplier: G-Biosciences
A 10X concentrated Tris Buffered Saline supplemented with Tween 20.
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Phosphate-buffered saline (PBS, 10X), RNase free
Supplier: Thermo Fisher Scientific
Cell culture buffer, PBS, 10 X, RNAse free
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Succinate, 0,2 M buffer solution, pH 6,5
Supplier: Thermo Fisher Scientific
Succinate buffer solution, 0,2 M, pH value: 6,5, Colourless, Odourless, Liquid
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Buffers, potassium phosphate, 0,5 M solutions
Supplier: Thermo Fisher Scientific
Potassium phosphate, buffer solution, 0,5 M, pH value: 7,0, Liquid
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Buffers, TRIS, 1,0 M solutions
Supplier: Thermo Fisher Scientific
Odourless, colourless TRIS buffer solution.
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Buffer solution for chlorine determination
Supplier: Hach
For determination of chlorine by amperometric titration. APHA formulation 100 ml bottle.
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HEPES lysis buffer with Triton® X-100
Supplier: Thermo Fisher Scientific
HEPES lysis buffer with Triton® X-100, Thermo Fisher Scientific
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HEPES-Buffered Saline, 5X Solutions
Supplier: Thermo Fisher Scientific
HEPES buffered saline, 5X, pH value: 7,0, Odourless, Liquid
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#: 20864.320
Supplier: VWR Chemicals
#: 83640.320
Supplier: VWR Chemicals
#: 31770.294
Supplier: VWR Chemicals
#: 1.00030.2500
Supplier: Merck
#: 83645.320
Supplier: VWR Chemicals
#: 20060.320
Supplier: VWR Chemicals