Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Phosphate, 0,5M buffer solution, pH 9,0

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,5 M, pH value: 9,0, Liquid

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Buffer Solutions

Supplier: Thermo Fisher Scientific

pH Buffer solution, pH value: 8,00, ±0.01, Reference temperature: 25 °C

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Acetate buffer solution, Fluka™

Supplier: Honeywell Chemicals

This pH buffer solution is a combination of sodium acetate/acetic acid in 1:1 ratio for complexometry.

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DNA gel loading buffer 6X

Supplier: LONZA

This buffer is a Ficoll based loading buffer containing Ficoll®, EDTA, xylene cyanol, and bromophenol blue. Ideal for separation of DNA fragments with improved resolution.

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RIPA Lysis and Extraction Buffer

Supplier: G-Biosciences

A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many applications, including reporter assays, protein assays, immunoassays and other protein purification techniques.

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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer), MP Biomedicals

Supplier: MP Biomedicals

Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)

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Buffers, sodium borate, 0,5 M solutions

Supplier: Thermo Fisher Scientific

Sodium borate buffer solution, 0,5 M, pH value: 9,0, Colourless, Liquid

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Buffer kit for antibody purification on protein A column

Supplier: Thermo Fisher Scientific

Buffer kit for antibody purification

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Buffers, AMPSO, 0,2 M solutions

Supplier: Thermo Fisher Scientific

AMPSO buffer solution, 0,2 mol/L, pH value: 9,0, Colourless, Odourless, Liquid

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Protein extraction reagent, BugBuster® 10X, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.

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Buffers, TRIS, 1,0 M solutions, 0,2 µm filtered

Supplier: Thermo Fisher Scientific

Colourless odourless TRIS buffers filtered.

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Buffer, HEPES solution (1 mol/L), Corning®

Supplier: Corning

HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulphonic acid) provides an alternative to the use of bicarbonate for buffering because it does not require elevated levels of CO2

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MES-buffered saline (5X), pH 6.5

Supplier: Thermo Fisher Scientific

MES-buffered saline, 5X, pH value: 6,5, Liquid

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Phosphate, 0,2 M buffer solution, pH 7,4

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,2 M, pH value: 7,4, Liquid

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Buffer, bicarbonate, 1M solution, pH 9,0

Supplier: Thermo Fisher Scientific

Bicarbonate, 1M buffer solution, pH 9,0, Liquid

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Sodium acetate, Corning® 3M™

Supplier: Corning

Sodium Acetate is a common molecular biology reagent. All production lots are tested to ensure that they are free of RNAse. It is often used in buffer systems to maintain the pH of a given solution.