Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Citrate-buffered saline (20X)

Supplier: Thermo Fisher Scientific

Saline-sodium citrate (SSC) buffer.

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Tricine-buffered saline (5X), pH 7,0

Supplier: Thermo Fisher Scientific

Tricine-buffered saline, 5X, pH value: 7,0, Colourless, Odourless, Liquid

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Sodium pyrophosphate, 200 mM buffer solution

Supplier: Thermo Fisher Scientific

Cloning Buffer, Sodium pyrophosphate, 200mM buffer solution

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Buffer, MOPS solution 10X concentrate

Supplier: LONZA

Running Buffer, MOPS buffer solution 10X concentrate

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Transfer buffer, Tris-glycine,10X, AccuGENE™

Supplier: LONZA

AccuGENE™ electrophoresis buffers are ready-to-use solutions ideal for a wide range of molecular biology applications.

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Blocking reagents, Blotto

Supplier: Rockland Immunochemicals

Contains immunoanalytical grade non-fat dry milk. Blocks non specific binding of proteins in various immunological assays. Ideal for use in western blotting and ELISA methods.

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Phosphate buffered saline, PBS-TWEEN® tablets, Millipore®

Supplier: Merck Millipore (Oncogene)

Developed for use in immunoassay applications.

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Buffers, BICINE, 0,5 M solutioins

Supplier: Thermo Fisher Scientific

N,N-(2-Dihydroxyethyl)glycine (BICINE) buffer solutions.

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TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 20X), with 0,5% Tween 20

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PBS, 10X solution, molecular biology grade, ultrapure

Supplier: Thermo Fisher Scientific

Washing buffer, PBS, 10X solution, molecular biology grade, ultrapure

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Buffers, TRIS, 0,5 M solutions

Supplier: Thermo Fisher Scientific

TRIS buffer solution, 0,5 mol/L, pH value: 8,6, Liquid

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Buffer, SSC 20X powder for molecular biology, MP Biomedicals

Supplier: MP Biomedicals

SSC buffer used in electrophoresis and in sedimentation studies of DNA-histone aggregates. SSC is used as a hybridisation buffer in immunoblotting and DNA microarray protocols.