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Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

VWR® Buffer Tablets for Haematology

VWR® Buffer Tablets for Haematology

Supplier: VWR Chemicals

These tablets provide a rapid and convenient method of preparing a buffer solution.

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VWR®, Tris buffer solution, pH 8 (1 mol/L) for biotechnology

VWR®, Tris buffer solution, pH 8 (1 mol/L) for biotechnology

Supplier: VWR Chemicals

Tris (hydroxymethyl) aminomethane.

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VWR® Transfer Buffer Concentrate, CAPS 10X

VWR® Transfer Buffer Concentrate, CAPS 10X

Supplier: VWR Chemicals

For transfer of protein to membranes for sequencing or blotting.

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VWR® Ultra Pure Grade, Sm buffer

VWR® Ultra Pure Grade, Sm buffer

Supplier: VWR Chemicals

Cloning Buffer, SM BUFFER

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VWR®, MOPS 10X buffer for biotechnology

VWR®, MOPS 10X buffer for biotechnology

Supplier: VWR Chemicals

MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a pH buffering range from 6,5 to 7,9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.

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VWR® UltraPure, Buffer, PBS solution 10X concentrate (phosphate buffered saline), USP

VWR® UltraPure, Buffer, PBS solution 10X concentrate (phosphate buffered saline), USP

Supplier: VWR Chemicals

Cell culture buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)

VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)

Supplier: VWR Chemicals

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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VWR® UltraPure, Tris-acetate-EDTA (TAE) buffer

VWR® UltraPure, Tris-acetate-EDTA (TAE) buffer

Supplier: VWR Chemicals

TAE is an extensively used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE buffer is more compatible with in-gel manipulations and band recovery procedures than TBE buffer.

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VWR®, EDTA buffer solution pH 8,0 (0,5 mol/L) for biotechnology

VWR®, EDTA buffer solution pH 8,0 (0,5 mol/L) for biotechnology

Supplier: VWR Chemicals

Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l) for biotechnology, sterile

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VWR®, SSC buffer, 20X, ultra-pure grade

VWR®, SSC buffer, 20X, ultra-pure grade

Supplier: VWR Chemicals

SSC buffer is a hybridisation buffer for Northern and Southern blots.

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VWR® TBE Buffer Solution, 10X Concentrate (Tris-Borate-EDTA Buffer), UltraPure Grade

VWR® TBE Buffer Solution, 10X Concentrate (Tris-Borate-EDTA Buffer), UltraPure Grade

Supplier: VWR Chemicals

Running Buffer, TBE buffer solution 10X concentrate (Tris-borate-EDTA buffer)

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VWR® Buffer, HEPES Solution pH 7,3 (1 mol/L)

VWR® Buffer, HEPES Solution pH 7,3 (1 mol/L)

Supplier: VWR Chemicals

HEPES buffer solution pH 7.3 (1 mol/l), VWR®

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VWR® Phosphate Buffered Saline (PBS), High Phosphate

VWR® Phosphate Buffered Saline (PBS), High Phosphate

Supplier: VWR Chemicals

Irradiated solution, pH 7,2, for washing blots.

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VWR® Tris Buffer Solution, pH 7,5 (1 mol/L), UltraPure Grade

VWR® Tris Buffer Solution, pH 7,5 (1 mol/L), UltraPure Grade

Supplier: VWR Chemicals

TRIS buffer solution pH 7,5 (1 mol/l), VWR®

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VWR®, Tris buffer solution, pH 9 (1 mol/L), ultrapure

VWR®, Tris buffer solution, pH 9 (1 mol/L), ultrapure

Supplier: VWR Chemicals

Cloning Buffer, TRIS buffer solution pH 9 (1 mol/l)

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VWR® Sodium Dihydrogen Phosphate Buffer Solution (Sodium dihydrogen orthophosphate/Phosphoric acid) for Chlorine Determination in Water Monitors

VWR® Sodium Dihydrogen Phosphate Buffer Solution (Sodium dihydrogen orthophosphate/Phosphoric acid) for Chlorine Determination in Water Monitors

Supplier: VWR Chemicals

Sodium dihydrogen phosphate buffer solution, Phosphate, pH value: 2,30, ±0,05, VWR®, Neutral, Plastic container

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VWR® Sodium Acetate Buffer 3,0 M Solution, pH 5,2

VWR® Sodium Acetate Buffer 3,0 M Solution, pH 5,2

Supplier: VWR Chemicals

With diethyl pyrocarbonate (DEPC).

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VWR® UltraPure, Tris-glycine buffers, 10X concentrate

VWR® UltraPure, Tris-glycine buffers, 10X concentrate

Supplier: VWR Chemicals

Running buffers for both 1-D and 2-D protein gel electrophoresis.

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VWR®, TE (Tris-EDTA) buffer, pH 8,0 for biotechnology

VWR®, TE (Tris-EDTA) buffer, pH 8,0 for biotechnology

Supplier: VWR Chemicals

Ready to use Tris-EDTA buffer.

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VWR® AVS TITRINORM, Cesium chloride/aluminium nitrate buffer solution acc. to schuhknecht and schinkel for determination of na, k and li by flame aas
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VWR® Lysis Buffer, Hypotonic

VWR® Lysis Buffer, Hypotonic

Supplier: VWR Chemicals

Detergent-free buffer for isolation of proteins from tissue culture cells. Cell lysis and the subsequent isolation of the crude cytoplasmic fraction completed in less than 30 m.

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VWR® Separating Gel Buffer, 4X

VWR® Separating Gel Buffer, 4X

Supplier: VWR Chemicals

4X concentrated buffer solution (1,5 M Tris, pH 8,8 and 0,4% SDS). Used for preparation of standard acrylamide:bis-acrylamide gels as well as preparation of gels using PAGE-PLUS™ acrylamide solution.

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VWR® Phosphate Buffered Saline (PBS), Low Phosphate, 10X

VWR® Phosphate Buffered Saline (PBS), Low Phosphate, 10X

Supplier: VWR Chemicals

Irradiated concentrate of PBS, suitable for use in washing cells after dilution.

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VWR® UltraPure, PBS and TBS wash buffers

VWR® UltraPure, PBS and TBS wash buffers

Supplier: VWR Chemicals

Wash buffers ideal for use in applications such as Western blotting and ELISA. Prevents high background by effectively removing unbound reagents.

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VWR® Gentle ReView™, Stripping Buffer

VWR® Gentle ReView™, Stripping Buffer

Supplier: VWR Chemicals

Gentle ReView™ Stripping Buffer is a mild, yet effective method for stripping primary and secondary antibodies from PVDF or nitrocellulose membranes.

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VWR®, Hybridisation buffers with 50% formamide

VWR®, Hybridisation buffers with 50% formamide

Supplier: VWR Chemicals

Hybridisation cocktail offering blocking and hybridisation for optimised complimentary base pairing of DNA and RNA in Southern blot, Northern blot, dot blot, colony and in situ hybridisation.

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VWR®, Tris-borate-EDTA (TBE) buffer

VWR®, Tris-borate-EDTA (TBE) buffer

Supplier: VWR Chemicals

TBE is optimised for DNA electrophoresis techniques using both acrylamide and agarose. Buffers are available as liquid concentrate or Ready-Pack™ (a foil pouch containing sufficient material to prepare 1 litre of 10X concentrate).

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VWR® A.C.E.™, Sequencing buffers

VWR® A.C.E.™, Sequencing buffers

Supplier: VWR Chemicals

A.C.E.™ Sequencing Buffers are optimised buffers for DNA capillary electrophoresis sequencing that provide excellent results like long read lengths, high resolution, and consistent run times.

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VWR® Phosphate Buffered Saline (PBS), Low Phosphate

VWR® Phosphate Buffered Saline (PBS), Low Phosphate

Supplier: VWR Chemicals

Irradiated solution for use in washing cells.

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VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade

VWR® Buffer, MOPS-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

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