Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Triethylammonium bicarbonate buffer, for HPLC, LiChropur™, Supelco®
Supplier: Merck
1M Buffer, also for use in ion-exchange chromatography and electrophoresis.
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Buffer, TRIS solution pH 7,6 (1 mol/L), Sigma-Aldrich®
Supplier: Merck
Cloning Buffer, TRIS buffer solution pH 7,6 (1 mol/l)
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Buffer, TAE solution 10X concentrate (TRIS-acetate-EDTA buffer), Sigma-Aldrich®
Supplier: Merck
Running Buffer, TAE buffer solution 10X concentrate (TRIS-acetate-EDTA buffer)
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Wash Solution Concentrate, 20X
Supplier: SERACARE LIFE SCIENCES
Convenient 20X concentrate containing imidazole-buffered saline and Tween® 20.
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Buffer, TRIS-tricine-SDS solution 10X concentrate, Sigma-Aldrich®
Supplier: Merck
Running Buffer, TRIS-tricine-SDS buffer solution 10X concentrate
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Phosphate buffer solution, BioUltra, Supelco®
Supplier: Merck
Phosphate buffer, BioUltra, >0,044 M Na₂HPO₄, >0,022 M NaH₂PO₄, pH value: 7,2
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Phosphate Buffered Saline with 0,05% TWEEN® 20, pH 7,4
Supplier: Merck
Phosphate buffered saline with 0,05% Tween® 20 or PBST contains PBS buffer with a low-concentration of Tween® detergent. It is a common wash buffer and due to the presence of detergent, it can easily remove or block excess components thus reducing foaming and non-specific binding on the membrane and microtiter plate wells.
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pH buffer solutions, NIST/DIN
Supplier: Mettler - Toledo
The NIST/DIN pH buffers are specified to three decimal places, e.g. 9.180, offering the highest possible pH precision.
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Phosphate buffered saline (PBS), tablet, Sigma-Aldrich®
Supplier: Merck
Phosphate buffered saline (PBS) is a buffer solution used in biological research. It is a water based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate.
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Buffers, concentrated solutions
Supplier: CHEMPUR
pH Buffer, concentrate, Phosphate, pH value: 6,80, Bottle
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Buffer solutions, ready to use, 2 to 6,9 pH range
Supplier: CHEMPUR
pH Buffer solution, Citrate, pH value: 4,00, ±0,05, Colourless, Bottle
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Buffer, Trizma® hydrochloride solution, TRIS solution pH 7,4 (1 mol/L), BioPerformance Certified, Sigma-Aldrich®
Supplier: Merck
Cloning Buffer, TRIS buffer solution pH 7,4 (1 mol/l)
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Ammonia - Ammonium Chloride Buffer Solution, pH 10
Supplier: CPACHEM
Ammonia - Ammonium chloride buffer solution.
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Tris-EDTA buffer solution, pH 8,0, for molecular biology, BioUltra, Sigma-Aldrich®
Supplier: Merck
This buffer is 10 mM Tris-HCl and 1 mM disodium EDTA.
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Buffer, TRIS solution pH 7,5 (1 mol/L), Sigma-Aldrich®
Supplier: Merck
Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)
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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Sigma-Aldrich®
Supplier: Merck
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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