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You searched for: Assays

Assays

An assay is an investigative procedure in laboratory medicine, pharmacology, environmental biology, and molecular biology for qualitatively assessing or quantitatively measuring the presence or functional activity of the analyte, or the entity being targeted by the assay. Generally, assays involve biological materials or phenomena which tend to be intrinsically more complex in composition or in behavior than what can be handled by traditional chemical analysis and titration.

FLUOR DE LYS® HDAC1 fluorometric drug discovery assay kit

Supplier: ENZO LIFE SCIENCES

The HDAC1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC1 included in the kit.

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LDH Cytotoxicity WST Assay

Supplier: ENZO LIFE SCIENCES

Colorimetric kit for the determination of cytotoxicity through the measurement of lactate dehydrogenase activity.

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Fluor-de-Lys® SIRT2 fluorometric drug discovery assay kit

Supplier: ENZO LIFE SCIENCES

A Fluor-de-Lys® fluorescent assay system. The SIRT2 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT2 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.

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Granzyme B assay kit for drug discovery

Supplier: ENZO LIFE SCIENCES

The Granzyme B Assay Kit for Drug Discovery is a complete assay system designed to measure the protease activity of granzyme B. It contains a colorimetric substrate (IEPD-pNA) to measure this activity; cleavage of the p-nitroanilide (pNA) group from the colorimetric substrate increases absorption at 405 nm.

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LDH Cytotoxicity Assay Kit

LDH Cytotoxicity Assay Kit

Supplier: Cayman Chemical

For measurement of LDH activity as an indicator of cell death.

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GOLGI ID® Green assay kit

Supplier: ENZO LIFE SCIENCES

The GOLGI ID® Green assay kit contains a Golgi apparatus-selective dye suitable for live cell, or aldehyde-fixed cell staining. It is more faithfully localized to the target organelle, with minimal staining of the endoplasmic reticulum. Micromolar concentrations of GOLGI ID® Green dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line, Jurkat, canine kidney cell line MDCK, and human bone osteosarcoma epithelial cell line U2OS.

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Caspase-1 assay kit for drug discovery

Supplier: ENZO LIFE SCIENCES

This kit is a complete assay system to measure protease activity of recombinant caspase-1. Cleavage of a tetrapeptide substrate is monitored colorimetrically at 405 nm or fluorimetrically (Ex. 360 nm/Em. 460 nm).

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Apoptosis assay kit, NucView® 488 and MitoView™ 633

Apoptosis assay kit, NucView® 488 and MitoView™ 633

Supplier: Biotium

NucView® 488 and MitoView™ 633 kit allows profiling of apoptotic cells simultaneously with changes in mitochondrial membrane potential using either fluorescence microscopy or flow cytometry.

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FLUOR DE LYS®-Green HDAC2 fluorometric drug discovery assay kit

Supplier: ENZO LIFE SCIENCES

The HDAC2 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC2 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or kinetic assay of the enzyme under varying conditions.

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APO-BRDU™ kit, TUNEL Assay

APO-BRDU™ kit, TUNEL Assay

Supplier: Tonbo Biosciences

The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.

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PROTEOSTAT® protein aggregation assay

PROTEOSTAT® protein aggregation assay

Supplier: ENZO LIFE SCIENCES

PROTEOSTAT® Protein aggregation assay provides a simple, homogenous assay format for monitoring peptide and protein aggregation in solution. This is useful for defining optimal storage formulations for proteins, for screening of compounds that promote or inhibit protein aggregation and potentially for the sensitive measurement of molecular chaperone activity. The assay can be employed to streamline protein processing and formulation optimization procedures.

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FLUOR DE LYS® HDAC fluorometric activity assay kit

Supplier: ENZO LIFE SCIENCES

The FLUOR DE LYS® HDAC fluorometric activity assay kit is a sensitive and convenient alternative to protocols utilizing radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. It is based on the unique FLUOR DE LYS® (Fluorimetric Histone deAcetylaseLysyl) substrate and developer combination and provides an assay that can be carried out in two simple mixing steps, all on the same 96-well plate. Histone deacetylase inhibitors have shown promise as anti-tumor agents and naturally this has stimulated interest in the screening of compounds for HDAC inhibition.

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LEADING LIGHT® Wnt Reporter Assay Starter Kit

Supplier: ENZO LIFE SCIENCES

The LEADING LIGHT® Wnt Reporter Assay Starter Kit is a cell-based luciferase activity test suitable for 96 well plate format.

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Caspase-2 fluorometric assay kit

Supplier: ENZO LIFE SCIENCES

Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate VDVAD-AFC.

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Steady-Luc™ Firefly HTS assay kit

Steady-Luc™ Firefly HTS assay kit

Supplier: Biotium

Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.

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Caspase-3 assay kits, NucView™ 488

Caspase-3 assay kits, NucView™ 488

Supplier: Biotium

NucView® 488 caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.

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Protease Estimation Assay Kit, ProteSEEKER™

Protease Estimation Assay Kit, ProteSEEKER™

Supplier: G-Biosciences

ProteSEEKER™ identifies specific types of proteases with a panel of twelve protease inhibitors and a sensitive colorimetric protease screening assay. ProteSEEKER™ allows researchers to screen their protein samples and establish which specific class of proteases are present and therefore design a highly specific protease inhibitor cocktail using the minimal number of protease inhibitors. Alternatively, ProteSEEKER™ can be used to test existing protease inhibitor cocktails and identify their inadequacies and therefore supplement in additional protease inhibitors.

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Cytochrome C releasing apoptosis assay kit

Supplier: ENZO LIFE SCIENCES

For the detection of Cytochrome c translocation from mitochondria into cytosol during apoptosis.

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ProteoStat™ thioredoxin-1 assay kit for microplates

Supplier: ENZO LIFE SCIENCES

This kit provides a simple, homogenous assay for screening modulators of Thioredoxin-1 enzymatic activity.

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ALDetect™ Lipid Peroxidation Assay kit

Supplier: ENZO LIFE SCIENCES

The assay is designed to measure either MDA alone (in hydrochloric acid) or MDA in combination with 4-hydroxyalkenals (in methanesulfonic acid). It is based on the reaction of a chromogenic reagent, N-methyl-2-phenylindole with MDA and 4-hydroxyalkenals to yield a stable chromophore with maximal absorbance at 586 nm.

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Glycolysis Cell-Based Assay Kit

Glycolysis Cell-Based Assay Kit

Supplier: Cayman Chemical

A colorimetric-based method for detecting L-lactate in culture medium.

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eFluxx-ID® multidrug resistance assay kit

Supplier: ENZO LIFE SCIENCES

The EFLUXX-ID® Multidrug Resistance Assay Kit allows for functional detection of all three clinically relevant ABC transporter proteins MDR1 (p-glycoprotein), MRP1/2, and BCRP. The assay uses a hydrophobic, non-fluorescent compound that readily penetrates the cell membrane, where it is hydrolyzed to a hydrophilic fluorescent dye by intracellular esterases.

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CFDA SE Cell proliferation assay kit

Supplier: Biotium

Cell proliferation dyes diffuse passively into cells and covalently label intracellular proteins, resulting in long term cell labelling. They are non-fluorescent until they are hydrolysed by intracellular esterase's. The dyes then react with intracellular amines forming fluorescent conjugates that are retained in the cell. Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry. With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labelling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analysed immediately after staining. Staining is formaldehyde fixable.

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Fluorometric HTS assay kits, Caspase-3 and Caspase-8

Fluorometric HTS assay kits, Caspase-3 and Caspase-8

Supplier: Biotium

Caspase-3 and Caspase-8 Fluorometric HTS Assay Kits provide a homogenous assay system for fast and highly sensitive detection of Caspase-3 or Caspase-8 activity by fluorescence in enzymatic reaction or mammalian cells.

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Superoxide dismutase assay kit

Superoxide dismutase assay kit

Supplier: Cayman Chemical

Superoxide Dismutase Assay (SOD) kit is a fast and reliable assay for the measurement of SOD activity from plasma, serum, tissue homogenates and cell lysates.

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Bacterial Ssaining, viability/cytotoxicity assay kit for bacteria live and dead cells

Supplier: Biotium

The Bacteria Viability/Cytotoxicity Assay Kit provides two-colour fluorescence staining of both live bacteria (green) and dead bacteria (red) using two probes, DMAO and EthD-III.

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LEADING LIGHT® Wnt cell line assay reagents

Supplier: ENZO LIFE SCIENCES

For use with the LEADING LIGHT® Wnt Reporter Cell Line.

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ALDetect™ (MDA-Specific) Lipid Peroxidation Assay kit

Supplier: ENZO LIFE SCIENCES

Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from polyunsaturated fatty acids, are unstable and decompose to form a complex series of compounds. These include reactive aldehydes, of which the most abundant is malondialdehyde (MDA). Therefore, measurement of malondialdehyde is widely used as an indicator of lipid peroxidation. Increased levels of lipid peroxidation products have been associated with a variety of chronic diseases in both humans and model systems.

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Steady-Luc™ Firefly HTS assay kit (lyophilised)

Steady-Luc™ Firefly HTS assay kit (lyophilised)

Supplier: Biotium

Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.

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FLUOR DE LYS® HDAC fluorometric cellular activity assay kit

Supplier: ENZO LIFE SCIENCES

The HDAC fluorometric cellular activity assay allows the determination of deacetylase activity within an undisturbed cellular environment and provides accurate activity information that is reflective of endogenous regulation. It also allows the study of the effects of upstream regulators on deacetylase activity and the detection of inhibitors or activators that act indirectly to affect deacetylase activity.

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