You searched for: Assays

Supplier: G-Biosciences

CB-X™ Protein Assay is a Coomassie dye based protein assay based on the Bradford Protein Assay. For protein samples in simple, uncomplicated aqueous buffers CB-X™ is a highly sensitive, single reagent assay that can be performed in 5 minutes. For complicated protein samples, CB-X™ Protein Assay is supplied with reagents to clean up the samples and remove all reagents and chemicals that interfere with accurate protein estimation in a single step. These reagents include detergents, chaotropes, reducing agents, alkylating agents, sugars, high salt concentrations, buffering agents and chelating agents. The clean up stage and subsequent protein assay is performed in a single tube to ensure no protein loss and to maintain the accuracy of the assay.

Supplier: Cayman Chemical

Triglyceride Colorimetric Assay Kit is a simple, reproducible and sensitive tool for assaying triglycerides in plasma, serum, cell lysates and tissue homogenate samples.

Supplier: Cayman Chemical

Phagocytosis Assay Kit (IgG FITC) for the identification of factors regulating the phagocytic process in vitro.

Supplier: Cayman Chemical

A simple, reproducible, and sensitive tool for assaying free thiol content in samples.

Supplier: Enzo Life Sciences

This kit is a complete assay system for measuring caspase-3 and caspase-3-like activity (DEVDase) in cellular extracts.

Supplier: Enzo Life Sciences

FluoForte® Calcium Assay Kits detect mobilisation of intracellular calcium utilising a fluorogenic calcium-binding dye optimised for superior cell-permeability and retention. The self-quenching dye undergoes an electronic change upon binding of calcium, resulting in a several order of magnitude greater fluorescence.

Supplier: Cayman Chemical

Cellular assay, Cell-based assay calcein AM

Supplier: G-Biosciences

This Protease Assay Kit is designed for the quantitative determination of proteases present in protein sample, using a dye-labelled protein substrate. The proteases present in the sample of interest will digest the protein substrate and release dye labelled peptides. The absorbance of the dye-labelled peptide is measured at 570 nm for determination of protease activity.

Supplier: G-Biosciences

The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample. The assay uses fluorescein isothiocyanate (FITC)-labelled casein as a general protease substrate. The fluorescein label on the FITC-casein is highly quenched. When the proteases present in the sample of interest digest the FITC-casein substrate into smaller peptides, the quenching of the fluorescence label is relieved and the fluorescence of the substrate is increased. The fluorescence of the FITC-labeled peptide is measured with excitation at 485 nm and emission at 530 nm to determine protease activity. The kit detects picogram level of proteases present in the sample.

Supplier: Cayman Chemical

A cell-based assay for studying modulators of cellular glucose uptake.

Supplier: Cayman Chemical

Cholesterol Cell-Based Detection Assay Kit is a 192-well assay for measurement of cholesterol.

Supplier: Biotium

This kit is designed to measure firefly luciferase activity in transfected cell reporter assays.

Supplier: Enzo Life Sciences

Histone acetyltransferases (HATs) have been implicated to play a crucial role in various cellular functions, such as gene transcription, differentiation, and proliferation. HAT Activity Colorimetric Assay Kit offers a convenient, nonradioactive system for a rapid and sensitive detection of HAT activity in mammalian samples.

Supplier: Biotium

MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.

Supplier: G-Biosciences

A highly sensitive colorimetric protein assay that overcomes interference of common laboratory agents present in protein solutions and shows minimal protein-to-protein variation. The assay is unaffected by the presence of common laboratory agents such as reducing agents, chelating agents, detergents, amines, sugars, chaotropes, salts, drugs, antibiotics, cobalt, and others.

Supplier: G-Biosciences

These CL (Compatible Lowry) Protein Assay is based on the widely cited protein assay by Lowry et. al. (1951). The CL Protein Assay improves upon the traditional Lowry method to be compatible with common laboratory agents known to interfere with Lowry protein assays such as reducing agents (dithiothreitol (DTT), ß-mercaptoethanol and TCEP), detergents, chelating agents, amines, sugars, strong chaotropic buffers, salts, drugs, antibiotics, cobalt and other common laboratory agents.

Supplier: Enzo Life Sciences

COMET SCGE Assay measures DNA damage by fluorescently detecting the integrity of DNA liberated from cells embedded in low melting point agarose. Upon electrophoresis, fragmented DNA produces a characteristic "comet" shaped tail as small DNA fragments migrate in the gel more rapidly than in-tact genomic DNA.

Supplier: Cayman Chemical

96-Well assay for measurement of p53.

Supplier: Enzo Life Sciences

Red Hydrogen Peroxide Assay Kit provides a simple absorbance-based or fluorometric assay to detect and quantify hydrogen peroxide or peroxidase activity in biochemical assays, cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized ‘mix and read’ assay that is suitable for both 96- and 348-well formats and is compatible with HTS liquid handling.

Supplier: Cayman Chemical

96-Well assay for measurement of Oct4.

Supplier: Biotium

ATP-Glo™ Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. The homogeneous assay involves a single addition of ATP-Glo™ Detection Cocktail directly to cells in culture medium.

Supplier: G-Biosciences

RED 660™ Protein Assay is a single reagent colorimetric assay that outperforms many commercial colorimetric assays, including Bradford and improved Coomassie/Bradford assays. It offers greater linearity, colour stability, and compatibility with detergents, reducing agents and other interfering agents compared to the Coomassie assays. The single, ready to use reagent allows for rapid analysis of total protein concentration and generates highly reproducible results.

Supplier: Enzo Life Sciences

Convenient method for the enhanced induction and detection of adipogenesis, in the classic 3T3L-1 model.

Supplier: Cayman Chemical

Uric Acid Assay Kit is used to measure uric acid in serum, plasma and urine.

Supplier: Cayman Chemical

A 96-well assay for measurement of SREBP-1.

Supplier: Cayman Chemical

For the determination of total calcium concentration in a variety of biological samples, as well as tissue homogenates and cell lysates.

Supplier: Cayman Chemical

Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.

Supplier: Enzo Life Sciences

The assay utilizes bioluminescent detection of the ATP level for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin. The light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well).

Supplier: Cayman Chemical

A 96-well assay for measurement of PPARδ DNA binding activity.

Supplier: Cayman Chemical

A 96-well assay for measurement of PPARα DNA binding activity.