Supplier: Enzo Life Sciences

Convenient method for the enhanced induction and detection of adipogenesis, in the classic 3T3L-1 model.

Supplier: Enzo Life Sciences

Simple assay to quantify senescence-associated β-galactosidase activity, in live cells, using flow cytometry or fluorescent microscopy.

Supplier: Enzo Life Sciences

The DPPIV/CD26 Assay kit for biological samples is a complete assay system designed to measure DPPIV activity in biological fluids such as plasma, serum, urine, and saliva. Uses for the kit include correlation of DPPIV activity with disease states or determination of the efficacy of DPPIV inhibitors administered in vivo.

Supplier: Enzo Life Sciences

This colorimetric kit is designed for inhibitor screening or kinetic assay of recombinant Calcineurin. Activity is determined using the RII phosphopeptide substrate, the most efficient and selective peptide known for calcineurin, and detection of free phosphate released is based on the classic malachite green assay.

Supplier: Cayman Chemical

A 96-well assay for measurement of SREBP-1.

Supplier: Cayman Chemical

For the determination of total calcium concentration in a variety of biological samples, as well as tissue homogenates and cell lysates.

Supplier: Cayman Chemical

A 96-well assay for measurement of PPARα DNA binding activity.

Supplier: Enzo Life Sciences

The GFP-Certified™ FluoForte™ Calcium Assay kit provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.

Supplier: Cayman Chemical

WST-1 Proliferation Assay Kit provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model. The assay is based on the reduction of tetrazolium salt WST-1 to soluble formazan by electron transport across the plasma membrane of dividing cells. This kit will also allow investigators to screen drug candidates involved in regulation of cell cycle.

Supplier: Enzo Life Sciences

The assay is designed to detect ADP/ATP ratio. The changes in ADP/ATP ratios have been used to conveniently differentiate apoptosis from necrosis.

Supplier: Enzo Life Sciences

A complete colorimetric assay kit for measuring cellular calcineurin (PP2B) phosphatase activity. It employs a convenient 96-well plate format with all reagents necessary for measuring calcineurin (PP2B) phosphatase activity in tissue/cellular extracts PLUS human recombinant calcineurin is included as a positive control.

Supplier: Enzo Life Sciences

The PROTEOSTAT® PDI assay kit provides a simple, homogenous assay for screening modulators of PDI enzymatic activity. This is accomplished by monitoring PDI-catalyzed reduction of insulin in the presence of Dithiothreitol (DTT), resulting in the formation of insulin aggregates which then bind avidly to the red-emitting fluorogenic PROTEOSTAT® PDI detection dye.

Supplier: Biotium

Calcein-AM is a non-fluorescent, membrane permeable compound. Esterase activity in the cytoplasm of viable cells converts calcein-AM to the green fluorescent, membrane impermeant compound calcein, which is retained in viable cells with intact plasma membranes. The Viability/Cytotoxicity Assay kit for Animal Live & Dead Cells pairs calcein-AM with the vital dye ethidium homodimer III for quantitation of live and dead cells.

Supplier: Cayman Chemical

The Protein Carbonyl Colorimetric Assay Kit is for the measurement of oxidised proteins. Protein samples are derivatised by making use of the reaction between 2,4-dinitrophenylhydrazine and protein carbonyls.

Supplier: Enzo Life Sciences

Colorimetric kit for the determination of cytotoxicity through the measurement of lactate dehydrogenase activity.

Supplier: Enzo Life Sciences

Cellular assay, APO-BRDU™ kit

Supplier: G-Biosciences

The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions.

Supplier: VWR Chemicals

The Bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. Based upon complex formation between basic and aromatic amino acid residues with Coomassie® Brilliant Blue G-250 dye, the Bradford method is easier, faster, and more sensitive than the Lowry method.

Supplier: G-Biosciences

CB™ Protein Assay, a Coomassie dye based protein assay based on the Bradford Protein Assay, is suitable for the simple and rapid estimation of protein concentration. The binding of protein to the dye results in a colour change from brown to blue. The change in colour density is proportional to protein concentration. Protein estimation can be performed using as little as 0,5 µg protein.

Supplier: Enzo Life Sciences

The GOLGI ID® Green assay kit contains a Golgi apparatus-selective dye suitable for live cell, or aldehyde-fixed cell staining. It is more faithfully localized to the target organelle, with minimal staining of the endoplasmic reticulum. Micromolar concentrations of GOLGI ID® Green dye are sufficient for staining mammalian cells, as validated with human cervical carcinoma cell line HeLa, human T-lymphocyte cell line, Jurkat, canine kidney cell line MDCK, and human bone osteosarcoma epithelial cell line U2OS.

Supplier: Biotium

This NucView™488 and RedDot™2 Apoptosis and Necrosis Assay Kit contains NucView™488 caspase-3 substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.

Supplier: Cayman Chemical

NF-κB (p65) Transcription Factor Assay Kit is a 96-well assay for measurement of NF-κB (p65).

Supplier: Enzo Life Sciences

The ER-ID® Green assay kit is specifically designed for use with RFP- or OFP-expressing cell lines, as well as cells expressing blue or cyan fluorescent proteins (BFPs, CFPs). Additionally, the kit is suitable for use with live or post-fixed cells in conjunction with probes, such as labeled antibodies, or other fluorescent conjugates displaying similar spectral properties as Texas Red, or coumarin. A nuclear counterstain is provided to highlight this organelle as well.

Supplier: Cayman Chemical

HIF-1α Transcription Factor Assay Kit is a sensitive, non radioactive method for detecting HIF-1α from whole cell lysates.

Supplier: Enzo Life Sciences

Highly reproducible SA-β-gal activity kit

Supplier: Enzo Life Sciences

ER-ID® Red assay kit (GFP-CERTIFIED®) contains an endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining.

Supplier: Enzo Life Sciences

BIOMOL® Green provides a simple and convenient method for colorimetric phosphate quantitation. It is suitable for applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase. It is most widely applied to protein phosphatase assays.

Supplier: Enzo Life Sciences

The LEADING LIGHT® Wnt Reporter Assay Starter Kit is a cell-based luciferase activity test suitable for 96 well plate format.

Supplier: Cayman Chemical

For measurement of LDH activity as an indicator of cell death.

Supplier: Biotium

NucView® 488 caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.