Nucleotides

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.

Supplier: Enzo Life Sciences

These ddUTPs are used for 3’-end labeling of oligodeoxynucleotides using terminal transferase. ddUTP also serves as a substrate for E. coli DNA Polymerase I (holoenzyme and Klenow fragment), T4 and T7 DNA polymerases, Taq DNA polymerase and reverse transcriptase.

Supplier: Enzo Life Sciences

Red dUTP's (Red 594 dUTP, Red 598 dUTP) can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.

Supplier: TriLink BioTechnologies

TriLink® 2'-O-Methyluridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

Biotin-16-Aminoallyluridine-5'-Triphosphate (Biotin-16-UTP, Biotin-16-AA-UTP) can be incorporated via a number of RNA polymerases, including SP6-, T3- and T7-RNA polymerase to form labeled RNA.

Supplier: Biotium

5-TAMRA-dUTP (5-Tetramethylrhodamine-dUTP) can be enzymatically incorporated into DNA to make fluorescently labeled probes, and has absorption/emission at 553/577 nm. Note: 5-TAMRA-dUTP may not be compatible with PCR labeling of DNA probes. Supplied as a 1 mM solution in Tris-HCl buffer, pH 7.5.

Supplier: TriLink BioTechnologies

TriLink® Biotin-7-Deaza-7-Propargyl-dGTP

Supplier: Enzo Life Sciences

Cyanine 3-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 3-dUTP can also be used for multicolor fluorescence labeling.

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.

Supplier: Enzo Life Sciences

Ultra-pure, stable, and suitable for a variety of PCR applications.

Supplier: TriLink BioTechnologies

TriLink® Uridine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

N1-Methyl-Pseudouridine-5'-Triphosphate is widely used in mRNA synthesis for therapeutic and vaccine development to improve mRNA functionality. This nucleotide consists of a modified base, a ribose, and a 5′ triphosphate group.

Supplier: Enzo Life Sciences

The procedure for labeling of DNA probes with a polynucleotide 'tail' containing hapten-labeled nucleotides was developed by Enzo. In such terminal labeling reactions, terminal transferase catalyzes the addition of nucleotides to any 3'-OH terminus in a template independent manner. This rapid and convenient nonradioactive labeling procedure is free of any sequence bias that is normally observed in random priming or nick translation reactions.

Supplier: TriLink BioTechnologies

TriLink® N7-Methyl-3'-O-Methyl-Guanosine-5'-Triphosphate-5'-Guanosine

Supplier: TriLink BioTechnologies

This deoxyguanosine analog lacks the N7 nitrogen found in the natural guanine nucleobase. Replacement of this nitrogen atom with a carbon atom results in diminished structure of the major groove in DNA and may disrupt base stacking, but does not interfere with Watson-Crick base pairing. This is desirable for applications such as amplification and sequencing of GC-rich nucleic acid sequences.

Supplier: TriLink BioTechnologies

TriLink® 2'-Deoxythymidine-5'-O-(1-Thiotriphosphate)