Assay Buffers
Assay buffers are mixtures of buffers, salts, detergents, protein, preservatives, and coloring agents. These buffers are used in ELISA assays for dilution of standards, samples, and reagents, and for blocking of non-specific interactions. Buffers may be used individually or in combination, Buffers may be used individually or in combination. Blocking buffers are specifically designed to preserve the conformational spatial arrangement of proteins in their native folded state, ensuring the biological activity of the protein.
Solubilisation buffer, 2D-Xtract™
Supplier: G-Biosciences
A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.
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