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691 results for Enzymes

 

Enzymes

Enzymes accelerate, or catalyze, chemical reactions, and they are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.

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Pyruvate kinase, MP Biomedicals

Supplier: MP Biomedicals

Dissolves readily at 5 mg/ml in 0,06 M Tris/HCI pH 7,4 to give a clear colorless solution.

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alpha-Chymotrypsin, MP Biomedicals

Supplier: MP Biomedicals

One unit will hydrolyse 1,0 µmole of N-benzoyl-L-tyrosine ethyl ester (BTEE) per minute at pH 7,8 and 25 °C in the presence of ionised calcium.

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Lipase, MP Biomedicals

Supplier: MP Biomedicals

One USP unit of lipase activity is contained in the amount of pancreatin that liberates 1,0 µEq of acid per minute at a pH of 9,0 at 37 °C under the conditions of the assay for lipase activity.

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Crotalus adamanteus Phosphodiesterase I, MP Biomedicals

Supplier: MP Biomedicals

Lyophilised in vials containing approximately 5 mg dry wt. and at least 100 units/vial (20 to 40 units/mg dry wt.).

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Pancreatin, Protease, Lipase, Amylase

Supplier: MERCK PRODUCTION CHEMICALS

Pancreatin 1400 FIP-U/g Protease, 24000 FIP-U/g Lipase, 30000 FIP-U/g Amylase EMPROVE® exp Ph. Eur., USP, ESSENTIAL

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Pig trypsin (from pancreas), MP Biomedicals

Pig trypsin (from pancreas), MP Biomedicals

Supplier: MP Biomedicals

Trypsin-EDTA solution is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation due to its digestive strength.

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Thrombin

Thrombin

Supplier: Cytiva

Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors containing the recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3).

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Microbe creatinine deiminase, MP Biomedicals

Supplier: MP Biomedicals

Creatinine deiminase has been used in a study to assess the application of a creatinine-sensitive biosensor for hemodialysis control. It has also been used in a study to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples.

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Sheep Hyaluronidase (from Testes), MP Biomedicals

Supplier: MP Biomedicals

Hyaluronidase is a glycoprotein containing 5% mannose and 2,17% glucosamine, it catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido- 2-deoxy- b-D-glucose and D-glucose residues in hyaluronate. It is a tetramer consisting of 4 equal subunits with a molecular mass of 14 kDa each.

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Streptococcus sp. glycerol-3-phosphate oxidase, MP Biomedicals

Supplier: MP Biomedicals

Glycerol-3-phosphate oxidase has been used for sensitive metabolite assays of starch and lipid synthesis, pyrophosphate, ATP, ADP, and most glycolytic intermediates in Arabidopsis seeds. It is a part of the dihydroxyacetone phosphate:glycerol-3-phosphate cycle in the bloodstream form of Trypanosoma brucei.

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Bovine hyaluronidase (from Testes), MP Biomedicals

Bovine hyaluronidase (from Testes), MP Biomedicals

Supplier: MP Biomedicals

Hyaluronidase is a glycoprotein containing 5% mannose and 2,17% glucosamine, it catalyses the random hydrolysis of 1,4-linkages between 2-acetamido- 2-deoxy- b-D-glucose and D-glucose residues in hyaluronate. Hyaluronidase from bovine testes is a tetramer consisting of 4 equal subunits with a molecular mass of 14 kDa each.

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Streptococcus (Lancefield group C strain) streptokinase, MP Biomedicals

Supplier: MP Biomedicals

Streptokinase catalyzes hydrolysis of amide linkages. Forms a 1:1 complex with plasminogen which is then converted to streptokinase-plasmin. Both streptokinase-plasminogen and streptokinase-plasmin complexes can activate plasminogen. As a result of its interaction with human or rabbit plasminogen, streptokinase undergoes specific fragmentation.

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Catalase, MP Biomedicals

Supplier: MP Biomedicals

One unit decomposes 1,0 µmole of hydrogen peroxide per minute at pH 7,0, 25 °C.

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Cholesterol oxidase, MP Biomedicals

Supplier: MP Biomedicals

CHOD is soluble in cold 50 mM potassium phosphate buffer, pH 7,0. Prepare solutions immediately before use.

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Lactase (from Aspergillus oryzae), MP Biomedicals

Supplier: MP Biomedicals

One unit will hydrolyse 1,0 µmole of o-nitrophenyl-beta-D-galactoside to o-nitrophenol per minute at pH 4,5 and 30 °C.

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Pig alpha-Amylase (from Pancreas), MP Biomedicals

Supplier: MP Biomedicals

One unit catalyses the liberation of 1 mmole reducing group (calculated as maltose per minute at pH 6,9 at 20 °C).

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Pig trypsin (from pancreas), MP Biomedicals

Supplier: MP Biomedicals

Trypsin 1:250 from pig pancreas is used to release adherent cells from tissue culture plates for passaging. It was used in the isolation and culture of human endothelial cells.

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Clostridium histolyticum Collagenase, Type I, MP Biomedicals

Supplier: MP Biomedicals

Collagenase is a protease which cleaves the triple-helical protein called collagen. It has extensive use in biological studies, where it is used to prepare isolated cell suspensions.

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Human Plasminogen, MP Biomedicals

Supplier: MP Biomedicals

Plasminogen is a single-chain glycoprotein found in human plasma and extracellular fluid. Certain activators, such as tissue plasminogen activator (tPA), convert plasminogen to its active form, plasmin. Plasminogen has been used to study its conversion into plasmin.

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Bovine trypsin (from pancreas), MP Biomedicals

Supplier: MP Biomedicals

Trypsin consists of a single chain polypeptide of 223 amino acid residues. It is a member of the serine protease family. It composed of two subunits, α-trypsin and β-trypsin. α-Trypsin is composed of two peptide chains and β-trypsin is composed of one chain.

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Acetyl coenzyme A trilithium salt trihydrate, MP Biomedicals

Supplier: MP Biomedicals

Acetyl-CoA is produced via beta-oxidation of fatty acids, via the metabolism of carbohydrates - glucose 6-phosphate to pyruvate to acetyl-CoA and via the catabolism of amino acids. Acetyl-CoA has a number of metabolic opportunities. It is metabolised in the tricarboxylic acid cycle to produce carbon dioxide, water and energy.

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Protease

Supplier: Abnova

Protease

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Benzonase®

Supplier: MERCK PRODUCTION CHEMICALS

Benzonase®

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Glutathione S-Transferase

Supplier: Abnova

Glutathione S-Transferase

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Invertase

Supplier: MERCK PRODUCTION CHEMICALS

Humectants for confectionery

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Recombinant Poly(A) Polymerase (from E. coli)

Supplier: Abnova

Recombinant Poly(A) Polymerase (from E. coli)

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Recombinant Alkaline Phosphatase (from E. coli)

Supplier: Abnova

Recombinant Alkaline Phosphatase (from E. coli)

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beta-Galactosidase, MP Biomedicals

Supplier: MP Biomedicals

Inhibitors: p-Chloromercuribenzoate, lodoacetamide, heavy metal ions (Zn²⁺, Fe²⁺, Zn²⁺, Cd²⁺, Cu²⁺, Pb²⁺, Ag⁺, Hg²⁺), Ionic Detergents (SDS, DAC, etc.). Contaminants: The preparation is practically free from other glycosidases (a-galactosidase, a-,b-glucosidase, a-,b-mannosidase, etc.) and proteinase. Principle: o-Nitrophenyl-b-D-galactopyranoside (ONPG) b-galactosidase > o-Nitrophenol (ONP) +D-Galactose. The appearance of o-nitrophenol is measured at 410 nm by spectrophotemetry. Thermal stability: below 50 °C (pH 7,3; 15 min) (Lit.), Optimum Temperature: 50 to 55 °C (Lit.).

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Pig Trypsin (from Pancreas), MP Biomedicals

Supplier: MP Biomedicals

Pig Trypsin (from Pancreas)

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Papain

Supplier: MERCK PRODUCTION CHEMICALS

Papain

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