Enzymes accelerate, or catalyze, chemical reactions, and they are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.
Pyruvate kinase, MP Biomedicals
Supplier: MP Biomedicals
Dissolves readily at 5 mg/ml in 0,06 M Tris/HCI pH 7,4 to give a clear colorless solution.
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alpha-Chymotrypsin, MP Biomedicals
Supplier: MP Biomedicals
One unit will hydrolyse 1,0 µmole of N-benzoyl-L-tyrosine ethyl ester (BTEE) per minute at pH 7,8 and 25 °C in the presence of ionised calcium.
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Lipase, MP Biomedicals
Supplier: MP Biomedicals
One USP unit of lipase activity is contained in the amount of pancreatin that liberates 1,0 µEq of acid per minute at a pH of 9,0 at 37 °C under the conditions of the assay for lipase activity.
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Crotalus adamanteus Phosphodiesterase I, MP Biomedicals
Supplier: MP Biomedicals
Lyophilised in vials containing approximately 5 mg dry wt. and at least 100 units/vial (20 to 40 units/mg dry wt.).
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Pancreatin, Protease, Lipase, Amylase
Supplier: MERCK PRODUCTION CHEMICALS
Pancreatin 1400 FIP-U/g Protease, 24000 FIP-U/g Lipase, 30000 FIP-U/g Amylase EMPROVE® exp Ph. Eur., USP, ESSENTIAL
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Pig trypsin (from pancreas), MP Biomedicals
Supplier: MP Biomedicals
Trypsin-EDTA solution is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation due to its digestive strength.
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Thrombin
Supplier: Cytiva
Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors containing the recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3).
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Microbe creatinine deiminase, MP Biomedicals
Supplier: MP Biomedicals
Creatinine deiminase has been used in a study to assess the application of a creatinine-sensitive biosensor for hemodialysis control. It has also been used in a study to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples.
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Sheep Hyaluronidase (from Testes), MP Biomedicals
Supplier: MP Biomedicals
Hyaluronidase is a glycoprotein containing 5% mannose and 2,17% glucosamine, it catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido- 2-deoxy- b-D-glucose and D-glucose residues in hyaluronate. It is a tetramer consisting of 4 equal subunits with a molecular mass of 14 kDa each.
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Streptococcus sp. glycerol-3-phosphate oxidase, MP Biomedicals
Supplier: MP Biomedicals
Glycerol-3-phosphate oxidase has been used for sensitive metabolite assays of starch and lipid synthesis, pyrophosphate, ATP, ADP, and most glycolytic intermediates in Arabidopsis seeds. It is a part of the dihydroxyacetone phosphate:glycerol-3-phosphate cycle in the bloodstream form of Trypanosoma brucei.
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Bovine hyaluronidase (from Testes), MP Biomedicals
Supplier: MP Biomedicals
Hyaluronidase is a glycoprotein containing 5% mannose and 2,17% glucosamine, it catalyses the random hydrolysis of 1,4-linkages between 2-acetamido- 2-deoxy- b-D-glucose and D-glucose residues in hyaluronate. Hyaluronidase from bovine testes is a tetramer consisting of 4 equal subunits with a molecular mass of 14 kDa each.
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Streptococcus (Lancefield group C strain) streptokinase, MP Biomedicals
Supplier: MP Biomedicals
Streptokinase catalyzes hydrolysis of amide linkages. Forms a 1:1 complex with plasminogen which is then converted to streptokinase-plasmin. Both streptokinase-plasminogen and streptokinase-plasmin complexes can activate plasminogen. As a result of its interaction with human or rabbit plasminogen, streptokinase undergoes specific fragmentation.
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Catalase, MP Biomedicals
Supplier: MP Biomedicals
One unit decomposes 1,0 µmole of hydrogen peroxide per minute at pH 7,0, 25 °C.
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Cholesterol oxidase, MP Biomedicals
Supplier: MP Biomedicals
CHOD is soluble in cold 50 mM potassium phosphate buffer, pH 7,0. Prepare solutions immediately before use.
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Lactase (from Aspergillus oryzae), MP Biomedicals
Supplier: MP Biomedicals
One unit will hydrolyse 1,0 µmole of o-nitrophenyl-beta-D-galactoside to o-nitrophenol per minute at pH 4,5 and 30 °C.
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Pig alpha-Amylase (from Pancreas), MP Biomedicals
Supplier: MP Biomedicals
One unit catalyses the liberation of 1 mmole reducing group (calculated as maltose per minute at pH 6,9 at 20 °C).
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Pig trypsin (from pancreas), MP Biomedicals
Supplier: MP Biomedicals
Trypsin 1:250 from pig pancreas is used to release adherent cells from tissue culture plates for passaging. It was used in the isolation and culture of human endothelial cells.
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Clostridium histolyticum Collagenase, Type I, MP Biomedicals
Supplier: MP Biomedicals
Collagenase is a protease which cleaves the triple-helical protein called collagen. It has extensive use in biological studies, where it is used to prepare isolated cell suspensions.
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Human Plasminogen, MP Biomedicals
Supplier: MP Biomedicals
Plasminogen is a single-chain glycoprotein found in human plasma and extracellular fluid. Certain activators, such as tissue plasminogen activator (tPA), convert plasminogen to its active form, plasmin. Plasminogen has been used to study its conversion into plasmin.
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Bovine trypsin (from pancreas), MP Biomedicals
Supplier: MP Biomedicals
Trypsin consists of a single chain polypeptide of 223 amino acid residues. It is a member of the serine protease family. It composed of two subunits, α-trypsin and β-trypsin. α-Trypsin is composed of two peptide chains and β-trypsin is composed of one chain.
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Acetyl coenzyme A trilithium salt trihydrate, MP Biomedicals
Supplier: MP Biomedicals
Acetyl-CoA is produced via beta-oxidation of fatty acids, via the metabolism of carbohydrates - glucose 6-phosphate to pyruvate to acetyl-CoA and via the catabolism of amino acids. Acetyl-CoA has a number of metabolic opportunities. It is metabolised in the tricarboxylic acid cycle to produce carbon dioxide, water and energy.
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Recombinant Poly(A) Polymerase (from E. coli)
Supplier: Abnova
Recombinant Poly(A) Polymerase (from E. coli)
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Recombinant Alkaline Phosphatase (from E. coli)
Supplier: Abnova
Recombinant Alkaline Phosphatase (from E. coli)
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beta-Galactosidase, MP Biomedicals
Supplier: MP Biomedicals
Inhibitors: p-Chloromercuribenzoate, lodoacetamide, heavy metal ions (Zn²⁺, Fe²⁺, Zn²⁺, Cd²⁺, Cu²⁺, Pb²⁺, Ag⁺, Hg²⁺), Ionic Detergents (SDS, DAC, etc.). Contaminants: The preparation is practically free from other glycosidases (a-galactosidase, a-,b-glucosidase, a-,b-mannosidase, etc.) and proteinase. Principle: o-Nitrophenyl-b-D-galactopyranoside (ONPG) b-galactosidase > o-Nitrophenol (ONP) +D-Galactose. The appearance of o-nitrophenol is measured at 410 nm by spectrophotemetry. Thermal stability: below 50 °C (pH 7,3; 15 min) (Lit.), Optimum Temperature: 50 to 55 °C (Lit.).
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Pig Trypsin (from Pancreas), MP Biomedicals
Supplier: MP Biomedicals
Pig Trypsin (from Pancreas)