Use of a Novel Cell Lysis Buffer for Protection of AAVs and Enhancing Manufacturing Efficiency

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The manufacturing of recombinant adeno-associated virus (rAAV) for use as gene therapies is a complex process that has proven difficult to scale commercially.

During manufacturing, intracellular rAAVs are extracted from the host production cells through chemical and/or mechanical lysis. This step presents various challenges in achieving the optimal yield of highly infectious particles. Avantor has developed a novel cell lysis buffer solution with enhanced properties for the manufacturing of rAAVs, which can lead to increased manufacturing efficiency.

In this presentation, we will describe the production and extraction of rAAVs produced in HEK293 cells using this novel cell lysis solution. We will also demonstrate the enhanced protection of the rAAVs against sheer stress when using this cell lysis buffer as compared to traditional methods.

Together, the enhanced properties and the ease of use, enable this novel cell lysis buffer to provide a more robust and efficient manufacturing process to produce rAAVs.

This presentation was given during BPI Europe, Vienna, May 17-19, 2022

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Presented by:

Dr. Nandu Deorkar

Ph.D., MBA, Senior Vice President, Research & Development – Biopharma Production, Avantor

Nandu Deorkar is Senior Vice President, Biopharma Production Research & Development for Avantor. He is responsible for innovation strategy and planning, and execution of new products and technology development. During his more than 25-year career in research & development, Dr. Deorkar has been leading teams working on various aspects of upstream and downstream bioprocessing, single use systems, chemical/polymer R&D, drug development, formulation, drug delivery technologies, process development, and technology transfer. He has published more than 30 articles and holds more than 20 patents. Dr. Deorkar received Ph.D. from Indian Institute of Technology, Mumbai, India and MBA in Marketing from Fairleigh Dickinson University, Madison, NJ, USA.

Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Our portfolio is used in virtually every stage of the most important research, development and production activities in the industries we serve. Our global footprint enables us to serve more than 225,000 customer locations and gives us extensive access to research laboratories and scientists in more than 180 countries. We set science in motion to create a better world.