A UHPLC/HPLC Method Development Strategy with Complementary Stationary Phases to Maximise Selectivity and Resolution

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Friday, July 12, 2019
16:00 to 16:45 CEST (Berlin, Paris, Madrid)

In this seminar, we review the importance of chromatographic selectivity in RPLC from a theoretical and practical perspective and how this relates to analyte resolution for method development. With an understanding of selectivity, and using a variety of chromatographic data, we discuss phase design principles and how it is possible to introduce functionality to enhance selectivity through mechanisms such as hydrophobicity, π-π, dipole-dipole and shape selectivity interactions.

An overview of the method development workflow is discussed. Based upon the key parameters to maximise selectivity, a systematic and optimised method development screening platform is described and an example related substances method development activity is illustrated using the complementary stationary phases with MeOH and MeCN solvents.

Presented by:

Alan McKeown

As an experienced chromatographer in his own right, Alan has been invited and delivered chromatography technical seminars in Europe, Asia, North America and South America at conferences, customer sites and organised events.
Alan has published various peer-reviewed articles and written different book chapters on separation science, chromatography and genotoxic impurities.

Alan is a Fellow of the Royal Society of Chemistry and a Chartered Chemist.

Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Our portfolio is used in virtually every stage of the most important research, development and production activities in the industries we serve. Our global footprint enables us to serve more than 300,000 customer locations and gives us extensive access to research laboratories and scientists in more than 180 countries. We set science in motion to create a better world.