Streamlined reversed-phase method development using combined column screening and software modelling approach

Register & watch

Friday, October 11, 2019
15:00 to 15:45 CEST (Berlin, Paris, Madrid)

The adoption of a structured approach to method development has the potential to provide significant savings in both development time and costs.

Column screening is a widely adopted strategy that explores selectivity – the key to analyte resolution. The most promising result from screening experiments may then be taken for further optimisation to produce the final analytical method. The use of LC modelling software has the potential to further refine method development at this final optimisation stage and provide a more informed and robust final method. The approach outlined in this talk combines both column screening and modelling software to provide an efficient protocol for reversed phase method development.

Initially, gradient scouting with six ACE reversed-phase columns is used to identify the optimum stationary phase and mobile phase conditions. Once the optimum combination is identified, additional gradient runs are performed and entered into ChromSword software to model the separation. Simulation experiments are then used to rapidly determine a final separation.

Presented by:

Alan McKeown

As an experienced chromatographer in his own right, Alan has been invited and delivered chromatography technical seminars in Europe, Asia, North America and South America at conferences, customer sites and organised events.
Alan has published various peer-reviewed articles and written different book chapters on separation science, chromatography and genotoxic impurities.

Alan is a Fellow of the Royal Society of Chemistry and a Chartered Chemist.

Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Our portfolio is used in virtually every stage of the most important research, development and production activities in the industries we serve. One of our greatest strengths comes from having a global infrastructure that is strategically located to support the needs of our customers. Our global footprint enables us to serve more than 225,000 customer locations and gives us extensive access to research laboratories and scientists in more than 180 countries. We set science in motion to create a better world.