Practical UHPLC: Selectivity and rapid method development, method translations and instrument transfers

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Wednesday, January 23, 2019
16:00 to 16:45 CEST (Berlin, Paris, Madrid)

This discussion explores UHPLC method development approaches from simple to complex, and how different variables influence selectivity for the chromatographer.

With an understanding of selectivity, and using a variety of chromatographic data, stationary phase design principles are explored including how different chemistries can help the analyst. This is illustrated using a related substances method development activity.

The principles of method translation (HPLC to UHPLC and vice-versa) is demonstrated. Transferring a gradient UHPLC method between 4 different instruments, (including 3 different UHPLC instrument vendors) is also shown with hints and tips for success. Finally, high resolution UHPLC separations are shown.

Presented by:

Alan McKeown

As an experienced chromatographer in his own right, Alan has been invited and delivered chromatography technical seminars in Europe, Asia, North America and South America at conferences, customer sites and organised events.
Alan has published various peer-reviewed articles and written different book chapters on separation science, chromatography and genotoxic impurities.

Alan is a Fellow of the Royal Society of Chemistry and a Chartered Chemist.

Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Our portfolio is used in virtually every stage of the most important research, development and production activities in the industries we serve. One of our greatest strengths comes from having a global infrastructure that is strategically located to support the needs of our customers. Our global footprint enables us to serve more than 225,000 customer locations and gives us extensive access to research laboratories and scientists in more than 180 countries. We set science in motion to create a better world.