Generating CRISPR cell models, an overview, challenges and latest technologies

Register & watch

Friday, November 29, 2019
17:00 to 18:00 CEST (Berlin, Paris, Madrid)

CRISPR/Cas9 is a revolutionary technology to irreversibly and accurately modify the genome.

To successfully perform genome editing it is essential to understand which techniques are available to deliver CRISPR/Cas9 components into cells. As transfection experts, Polyplus-transfection gives an overview of how CRISPR/Cas9 achieves precise genome editing, delves into key challenges and considerations that come with delivery of CRISPR/Cas9 components into cells, and presents latest technologies to address these challenges. The webinar will finish with a short Q&A session, with the opportunity for follow-up discussions.

Polyplus-transfection offers a large range of transfection reagents that cover available CRISPR approaches:

  • Transfection of plasmid DNA encoding Cas9, gRNA and gene of interest (jetPRIME and jetOPTIMUS)
  • Transfection of gRNA and mRNA encoding Cas9 (jetMESSENGER)
  • Delivery of RNP, meaning the Cas9 protein complexed with the gRNA (jetCRISPR and SpCas9 nuclease)

Presented by:

Alengo Nyamay'antu, PhD

Alengo Nyamay’antu is Scientific Support and Communication Specialist at Polyplus-transfection® SA, a leading biotechnology company that provides solutions for delivery of nucleic acids in research, bioproduction and therapeutics. Alengo completed her MSc in structural and functional biochemistry at the University of Lyon and went on to specialise in protein biochemistry at the University of Manchester. She then continued to develop and widen her scientific and communication skills by joining the Max Planck Institute for Biomedicine as a postdoctoral researcher. Alengo is particularly keen on exchanging and discussing science, and looks forward to the upcoming webinar to get you started on genome editing with the latest technologies.

Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. Our portfolio is used in virtually every stage of the most important research, development and production activities in the industries we serve. One of our greatest strengths comes from having a global infrastructure that is strategically located to support the needs of our customers. Our global footprint enables us to serve more than 225,000 customer locations and gives us extensive access to research laboratories and scientists in more than 180 countries. We set science in motion to create a better world.