Generating CRISPR cell models, an overview, challenges and latest technologies

CRISPR/Cas9 is a revolutionary technology to irreversibly and accurately modify the genome.

To successfully perform genome editing it is essential to understand which techniques are available to deliver CRISPR/Cas9 components into cells. As transfection experts, Polyplus-transfection gives an overview of how CRISPR/Cas9 achieves precise genome editing, delves into key challenges and considerations that come with delivery of CRISPR/Cas9 components into cells, and presents latest technologies to address these challenges. The webinar will finish with a short Q&A session, with the opportunity for follow-up discussions.

Polyplus-transfection offers a large range of transfection reagents that cover available CRISPR approaches:

• Transfection of plasmid DNA encoding Cas9, gRNA and gene of interest (jetPRIME and jetOPTIMUS)
• Transfection of gRNA and mRNA encoding Cas9 (jetMESSENGER)
• Delivery of RNP, meaning the Cas9 protein complexed with the gRNA (jetCRISPR and SpCas9 nuclease)