Challenges in Chromatography

Processing times can be reduced and yields increased by utilizing new hydrophobic interaction chromatography tools

By Dr Nandu Deorkar at Avantor

Advances in protein and peptide engineering continue, enabling the creation of new biological products with improved therapeutic and diagnostic potential. Bringing these promising biologics to market effectively depends, in part, on solving significant downstream purification process challenges – such as separation of closely related impurities – that are the result of the increased complexity of producing these biologics.

Platform-based chromatographic approaches have been successful in separating and purifying monoclonal antibody products, but the newer generations of biologics feature different hurdles for purification process developers to overcome. Product- and process-related impurities, including host cell protein, aggregates, and charge and glycosylation variants, are persistent and often difficult to separate. As a result, process developers are increasingly exploring mixed-mode and multimode chromatographic media to achieve these separations.

Continue reading article

Avantor® is a leading global provider of mission-critical products and services to customers in the life sciences and advanced technologies & applied materials industries. The company operates in more than 30 countries and delivers an extensive portfolio of products and services. As our channel brand, VWR offers an integrated, seamless purchasing experience that is optimized for the way our customers do business. We set science in motion to create a better world. For information visit, and find us on Linkedin, Twitter and Facebook.