81 Results for: "end point PCR enzymes and kits"

Supplier: Merck Millipore (Novagen)

Premixed 2X KOD Hot Start PCR components for convenience and reproducibility.

Supplier: Merck Millipore (Novagen)

KOD Xtreme™ Hot Start DNA Polymerase is an ultra high fidelity DNA polymerase designed for the most challenging PCR applications, including crude sample PCR, amplification of long strand and highly GC-rich DNA templates.

Supplier: Thermo Fisher Scientific

PCR Master Mix is a 2X concentrated solution of Taq DNA polymerase, dNTPs and all other components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimised for efficient and reproducible PCR.

Supplier: Merck Millipore (Novagen)

The NovaTaq PCR Master Mix is a ready to use 2X concentrated mixture of NovaTaq DNA polymerase, ultra-pure deoxynucleotides, and reaction buffer without magnesium chloride. The master mix simplifies the assembly of PCR reactions and offers advantages of times savings, consistency, and minimal risk of contamination. Simply add the NovaTaq PCR master mix to an equal volume containing the required amount of magnesium chloride, DNA template, and primers, and the reaction is ready for thermal cycling. The final diluted reaction contains 2,5 units of NovaTaq DNA polymerase per 100 µl. Sufficient components are included for 200 standard 50 µl (or 100×100 µl) amplification reactions.

Supplier: Merck Millipore (Novagen)

KOD HiFi DNA Polymerase is a unique proofreading enzyme, isolated from the extreme thermophile Thermococcus kodakaraensis KOD1, which possesses superior processivity and fidelity that enables faster and more accurate PCR amplification than can be achieved with conventional enzymes.

Supplier: FINNZYMES REAGENTS

Phusion® Hot Start II High-Fidelity DNA Polymerase allows the use of a wide range of primers, including those with low melting temperatures. Phusion® Hot Start II High-Fidelity DNA Polymerase is ideal for PCR applications from routine PCR to highly demanding applications, such as cloning and high throughput PCR. It incorporates a unique dsDNA-binding domain making this Pyrococcus-like proofreading DNA polymerase extremely robust, rapid, and accurate.

Supplier: FINNZYMES REAGENTS

Phusion® Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion® Hot Start II DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® Hot Start II DNA polymerase and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Supplier: G-Biosciences

Pfu DNA Polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerases. It has a molecular weight of 90 kDa and can amplify DNA targets up to 2 kb. The elongation velocity is 0,2~0,4 kb/min (70~75 °C). Pfu DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide misincorporation errors. This means that Pfu DNA Polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA Polymerase results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis.

Supplier: Cytiva

illustra™ Hot Start Mix, Ready-To-Go™ is a pre-formulated and pre-dispensed, freeze-dried PCR reagent mix, including high quality PuReTaq™ DNA polymerase and a hot start activator protein, for increased specificity and reproducibility of PCR amplifications. The Ready-To-Go™ beads are provided in either 0,5 or 0,2 ml tubes that are compatible with most thermal cyclers.

Supplier: FINNZYMES REAGENTS

Phusion® U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Supplier: FINNZYMES REAGENTS

Phusion® High Fidelity DNA Polymerase generates PCR products with an accuracy and speed unattainable with a single enzyme, even on the most difficult templates. The structure and characteristics of Phusion® High Fidelity DNA Polymerase make it a superior choice for cloning, and sets the standard for PCR performance with an error rate 50-fold lower than that of Taq DNA polymerase, and 6-fold lower than that of Pfu DNA polymerase. In addition, Phusion® High Fidelity DNA Polymerase possesses processivity 10-fold greater than Pfu DNA polymerase.

Supplier: FINNZYMES REAGENTS

Phusion® green hot start II high-fidelity PCR master mix is a convenient 2X mix designed to minimise the number of pipetting steps. The master mix contains Phusion Hot Start II DNA Polymerase, nucleotides and optimised reaction buffer including MgCl₂. The buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel.

Supplier: G-Biosciences

Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile Thermus aquaticus. The molecular weight of the recombinant protein is 94 kD. The Taq Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this Taq Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. Taq DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

Supplier: Thermo Fisher Scientific

Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents.

Supplier: FINNZYMES REAGENTS

Phusion® High-Fidelity PCR Kit offers extreme performance for all major PCR applications. Incorporating Phusion® DNA polymerase, the Phusion® High-Fidelity PCR Kit contains all the reagents necessary, including lambda DNA control template and primers for 1,3 kb and 10 kb amplicons. The template amount is sufficient for performing 20 control reactions in 50 μl volume or 50 control reactions in 20 μl volume.

Supplier: FINNZYMES REAGENTS

Phusion® Flash High-Fidelity PCR Master Mix is a 2X master mix enabling the use of extremely short PCR protocols without compromising either the fidelity or the yield in the reaction. The master mix utilises Phusion® Flash II DNA Polymerase, a proofreading DNA polymerase modified from Phusion® Hot Start II High-Fidelity DNA Polymerase. The fidelity of the Phusion® Flash II DNA Polymerase is 25-fold greater than Taq.

Supplier: MP Biomedicals

Taq-&GO™ is a ready to use optimised reaction mix for PCR.

Supplier: Thermo Fisher Scientific

Taq DNA Polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus. The enzyme catalyses 5'→3' synthesis of DNA, has no detectable 3'→5' exonuclease (proofreading) activity and possesses low 5'→3' exonuclease activity. In addition, Taq DNA polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products. Native Taq DNA polymerase is preferred for amplification of bacterial DNA sequences homologous to those found in E.coli. Native Taq DNA polymerase stabilised with BSA is often the best choice when amplifying DNA samples of lower purity, e.g. genomic DNA from mouse tail.

Supplier: Thermo Fisher Scientific

DreamTaq™ Green DNA Polymerase is a combination of DreamTaq™ DNA Polymerase and 10X DreamTaq™ Green Buffer. DreamTaq™ DNA Polymerase is an enhanced Taq DNA polymerase optimised for high throughput PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase.
DreamTaq™ Green DNA Polymerase incorporates modified nucleotides, but is inhibited by dUTP. The 10X DreamTaq™ Green Buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing,
phosphorylation, ligation and restriction digestion. For applications that require PCR product analysis by absorbance or fluorescence excitation, it is recommended either to use colourless 10X DreamTaq™ Buffer or purify the PCR product prior to analysis.

Supplier: FINNZYMES REAGENTS

Phusion® U hot start PCR master mix is a novel engineered high fidelity enzyme developed using fusion technology.

Supplier: EPPENDORF

Eppendorf Fast PCR tube strips made of polyethylene transfer heat faster than tubes made of traditional plastics. The Fast PCR tube strips are particularly suited to scientists using Fast PCR reagent kits, scientists trying to speed up protocols or cyclers with high heating and cooling rates.