Supplier: STEMCELL Technologies

Methylcellulose-based medium with recombinant cytokines for human cells. 1×100 ml bottle.

Supplier: STEMCELL Technologies

Membrane-permeable fluorescent probe for the detection of live neurons - 0,1 ml.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD38, FITC-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG2b antibody against human, rhesus HLA-DR, Alexa Fluor® 488-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human, chimpanzee CD22, PE­conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human, chimpanzee CD19, Alexa Fluor® 488-conjugated.

Supplier: STEMCELL Technologies

Medium for differentiation of human neural stem and progenitor cells.

Supplier: STEMCELL Technologies

cGMP, human T cell activation and expansion reagent.

Supplier: STEMCELL Technologies

Immunomagnetic isolation of untouched human pan dendritic cells (DC)

Supplier: STEMCELL Technologies

Immunomagnetic positive selection of particle-free mouse cells labelled with PE-conjugated antibodies.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human CD34, PE-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal antibody against mouse CD3, PerCP-Cy5.5-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal antibody against mouse, hamster CD4, PE-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal antibody against mouse, hamster CD4, PerCP-Cy5.5-conjugated.

Supplier: STEMCELL Technologies

Hamster (Armenian) monoclonal antibody against mouse T cell receptor beta, PE-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal antibody against mouse IL-4, unconjugated.

Supplier: STEMCELL Technologies

Rat monoclonal antibody against mouse CD127, APC-conjugated.

Supplier: STEMCELL Technologies

Rat (WI) monoclonal IgG2b antibody against mouse TER119.

Supplier: STEMCELL Technologies

A member of the SIBLING family of glycoproteins (Rangaswami et al.), Osteopontin (OPN) functions as T-helper 1 cytokine, and plays an important role in cell signaling, migration, and activation (Weber et al., 2002). Osteopontin has been shown to regulate inflammation both in vitro and in vivo (Agnholt et al.; Kiefer et al.), and has been implicated in the pathophysiology of numerous cancers, including prostate (Thalmann et al.), lung (Chambers et al.), ovarian (Kim et al.), and breast etiologies (Weber et al., 2015). In humans, OPN is initially secreted as a 317 amino acid protein which is subject to tissue-specific post-translational modifications by glycosylation, phosphorylation, and transglutamination (Kazanecki et al.; Sodek et al.). This protein product contains a His-residue tag at the amino end of the polypeptide chain. For consistency and reproducibility across your applications, Osteopontin from STEMCELL comes lyophilised with ≥92% purity, and endotoxin levels are verified to be ≤1,0 EU/μg protein.

Supplier: STEMCELL Technologies

Stimulating T cells with HPV16 (E6) Peptide Pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. HPV16 (E6) Peptide Pool is a lyophilised mixture of 37 peptides from the E6 protein of human papillomavirus type 16 (HPV16). The pool consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 158 on protein E6. E6 binds to the tumor suppressor protein p53 and stimulates its degradation (Scheffner et al.; Werness et al. ), which is thought to cause an increase in spontaneous mutagenesis in host cells (Havre et al.). E6 together with E7 demonstrated the ability to immortalize human keratinocytes in vitro, which likely has significance for HPVs oncogenic potential (Hawley-Nelson et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Use sclerostin to alter bone remodeling homeostasis, where it inhibits bone formation in vivo and in vitro, likely through Wnt/β-catenin signaling cascades (Ellies et al.; Lin et al.). Sclerostin is a member of the cerberus/DAN family of glycoproteins whose members share a C-terminal cysteine-knot-like (CTCK) domain. Highly expressed in bone and cartilage, as well as in kidney, and liver tissues (Weivoda et al.), this osteoclast-derived BMP antagonist binds BMP6 and BMP7 with high affinity, and binds BMP2 and BMP4 with a lower affinity (Kusu et al.). Mutations in the SOST gene have been associated with sclerosteosis (Brunkow et al.), van Buchem disease (Staehling-Hampton et al.), and bone dysplasia disorders, characterized by increased bone density. This protein product contains a His-residue tag at the carboxyl end of the polypeptide chain. For consistency and reproducibility across your applications, sclerostin from STEMCELL comes lyophilised with ≥87% purity, and endotoxin levels are verified to be ≤1.0 EU/μg protein.

Supplier: STEMCELL Technologies

Modulate prostaglandin metabolism with 15-hydroxyprostaglandin dehydrogenase (15-PGDH). 15-PGDH catalyses the reversible oxidation of the 15-hydroxyl group in prostaglandins, resulting in inactivated metabolites (Ensor and Tai), it can act on a variety of prostaglandins as substrates in a NAD+ dependent manner (Cho et al.). As prostaglandins can have a range of effects on cellular processes, 15-PGDH is of considerable importance in drug development. In vitro and in vivo studies suggest 15-PGDH has tumor suppressive activity and is downregulated in certain cancers (Na et al.).15-PGDH belongs to the short-chain dehydrogenases/reductases (SDR) family; its primary structure indicates 20% homology with other SDRs, with some conserved amino acid residues (Krook et al.). This protein contains a His-residue tag at the carboxyl end of the polypeptide chain. For consistency and reproducibility across your applications, apo M from STEMCELL comes lyophilised with ≥89% purity.

Supplier: STEMCELL Technologies

Fetuin B is thought to have functions in female fertility, metabolism, and liver disease. A protease inhibitor belonging to the fetuin family of proteins, within the cystatin superfamily, fetuin b’s structure includes two cystatin domains (CY1 and CY2) connected by a linker, with a CPDCP-trunk and a C-terminal region (CTR) (Cuppari et al.). This plasma protein originates in the liver (Denecke et al.) and acts to inhibit the activity of ovastacin, a metalloprotease that causes the zona pellucida to harden, leading to female infertility (Karmilin et al.). Fetuin B has also been reported to increase apoptosis, and inhibit migration and invasion of prostate cancer cells, in in vitro and in vivo settings (Zhan et al.). Elevated serum fetuin B has been linked to non-alcoholic fatty liver disease (NAFLD) (Zhu et al.). This protein contains a His-residue tag at the carboxyl end of the polypeptide chain. For consistency and reproducibility across your applications, apolipoprotein H from STEMCELL comes lyophilised with ≥92% purity, and is verified by LAL analysis to ensure endotoxin levels are ≤1,0 EU/μg protein.

Supplier: STEMCELL Technologies

Stimulating T cells with SARS-CoV-2 (Spike Protein) Omicron BA.4/BA.5 Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. This pool is provided as two lyophilised mixtures (subpools) from the spike glycoprotein of SARS-CoV-2 Omicron BA.4/BA.5 subvariant. The subpools contain 158 and 157 peptides, respectively, for a total of 315 peptides, and they consist of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 1270 on the spike protein. Through interactions with the angiotensin-converting enzyme 2 (ACE2) receptor, the spike protein enables the virus to attach to the cell membrane, and plays a critical role in viral entry (Hoffmann et al.; Walls et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Stimulating T cells with CMV (IE-1) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. CMV (IE-1) peptide pool is a lyophilised mixture of 120 peptides from the immediate early protein IE1 (IE1) of human cytomegalovirus (CMV; strain AD169), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 491 on IE1. CMV IE1 is a transcriptional transactivator and autoregulator (Cherrington and Mocarski). Studies show that IE1 promotes viral transcription by antagonizing histone deacetylation (Nevels et al.) and, along with IE2, may contribute to the survival of CMV-infected cells by blocking tumor necrosis factor alpha (TNF-α)-induced apoptosis (Zhu et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Myostatin is a member of transforming growth factor beta (TGF-β) superfamily that signals by binding to activin type II receptor, resulting in the recruitment of either ALK3 or ALK4 coreceptor, and activation of SMAD2/3 (Kondás et al.). It is released by myocytes and inhibits muscle growth and ability to regenerate (Lee and Lee). Myostatin appears to affect the lipid catabolic metabolism of adipocytes and inhibits preadipocyte differentiation, as demonstrated in the 3T3-L1 cell line (Li et al.).

Supplier: STEMCELL Technologies

Tumor necrosis factor-beta (TNF-β), also known as lymphotoxin-alpha, is a member of the TNF family. It is a pro-inflammatory cytokine that activates NF-kB, MAPK, and PI3K/AKT pathways upon binding to TNF receptors 1 and 2. It is produced mainly by T cells, though other cells can express TNF-β at lower levels (Chu). TNF-β is involved in autoimmune disorders, lymph node development, and mediating the inflammatory demyelination process (Seleznik et al.; McCarthy et al.).

Supplier: STEMCELL Technologies

Stimulating T cells with CMV (HLA Class I Control) Peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. CMV Peptide pool is a lyophilised mixture of 14 peptides from the human cytomegalovirus (CMV) and consists of defined HLA class I-restricted T cell epitopes. Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development. For consistency and reproducibility across your applications, CMV Peptide pools from STEMCELL are formulated as lyophilised trifluoroacetate salts, with a purity ≥95%.

Supplier: STEMCELL Technologies

Human (Actin) peptide pool is a lyophilised mixture of 92 peptides from human actin, alpha skeletal muscle, and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 377 on actin. It is frequently used as a negative control. Actin, alpha skeletal muscle, is part of the highly conserved actin protein family and is the predominant isoform found in adult skeletal muscle (Laing et al.). Peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

For detection and measurement of mouse interleukin 4.