2400 Results for: "STEMCELL Technologies"

Supplier: STEMCELL Technologies

Iscove's Modified Dulbecco's Medium (IMDM) with 25 mm HEPES.

Supplier: STEMCELL Technologies

Dulbecco's Modified Eagle's Medium (DMEM) with 4500 mg/l D-glucose.

Supplier: STEMCELL Technologies

Vascular endothelial growth factor (VEGF) is a heparin-binding homodimeric glycoprotein involved in vasculogenesis and angiogenesis. VEGF binds to FLT1 (VEGFR-1) and KDR (VEGFR-2), and activates Raf/MEK/ERK and PI3K/AKT pathways (Ferrara et al.). VEGF exists in multiple isoforms that result from alternative splicing of VEGF mRNA in the terminal exon. Proximal splice-site selection in exon 8 results in pro-angiogenic VEGFxxx isoforms (xxx is the number of amino acids), whereas distal splice-site selection results in anti-angiogenic VEGFxxxb isoforms (Nowak et al.). VEGF plays an important role in neurogenesis both in vitro and in vivo (Storkebaum et al.). It has neurotrophic effects on neurons of the central nervous system, and it promotes growth and survival of dopaminergic neurons and astrocytes. VEGF also promotes growth and survival of vascular endothelial cells, monocyte chemotaxis, and colony formation by granulocyte-macrophage progenitor cells (Ferrara et al.). Various splice variants of VEGF exist, with different functions. For example, it has been shown that VEGF isoform VEGF-164(165) and not VEGF-120(121) induces inflammation, stimulates intracellular adhesion molecule (ICAM)-1 expression on endothelial cells, and induces chemotaxis of monocytes (Usui et al.).

Supplier: STEMCELL Technologies

Interleukin 1 beta (IL-1β) is synthesized as an inactive precursor protein or pro-IL-1β. This precursor is cleaved intracellularly by caspase 1 (IL-1β convertase) to form the active form of the protein that is later secreted (Allan et al.). IL-1β binds to IL-1 receptor and activates intracellular signaling via the MAPK or NF-κB pathway. IL-1β is released by monocytes, tissue macrophages, and dendritic cells in response to infection or injury and induces expression of acute-phase proteins. It also promotes the infiltration of inflammatory and immunocompetent cells from the circulation into the extravascular space and affected tissues, by stimulating the expression of adhesion molecules on endothelial cells. IL-1β also affects other immune cells; for example, it co-stimulates T cell functions together with an antigen or mitogen. It also stimulates Th17 differentiation and B cell proliferation in an IL-6-dependent manner.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against rat NGFR/p75NTR (CD271), unconjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2a antibody against mouse Ly-6G, APC-conjugated.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2a antibody against human, mouse F4/80, PerCP-Cy5.5-conjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human, chimpanzee, horse CD38, unconjugated.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD2, unconjugated.

Supplier: STEMCELL Technologies

Interleukin 12 (IL-12p70) is a heterodimeric cytokine composed of p35 and p40 subunits. IL-12 is produced by monocytes, macrophages, dendritic cells, neutrophils and B cells in response to bacterial products and cytokines such as IFN-γ. The IL-12 receptor is expressed on T, NK, and dendritic cells. Upon binding, IL-12 initiates signaling via the JAK/STAT signaling pathway and stimulates NK, B, and T cells to produce IFN-γ (Watford et al.). It also regulates cytokine synthesis, proliferation of T and NK cells, and stimulates differentiation of CD4+ and CD8+ T cells (Germann and Rüde). Mice that are deficient in IL-12 are susceptible to many intracellular pathogens and have impaired IFN-γ secretion, Th1 differentiation and NK cytolytic activity; however, Th2 development and IL-4 production are enhanced (Watford et al.).

Supplier: STEMCELL Technologies

DAPI (4',6-diamidino-2-phenylindole dihydrochloride) is a blue-fluorescent dye that binds to AT-rich regions of double-stranded DNA.

Supplier: STEMCELL Technologies

Functional assay kit to assess pluripotency by directed differentiation of human ES and iPS cells to all three germ layers.

Supplier: STEMCELL Technologies

Serum-free neurophysiological basal medium for improved neuronal function.

Supplier: STEMCELL Technologies

Density gradient medium for use with RosetteSep™ myeloid cell enrichment cocktail.

Supplier: STEMCELL Technologies

Rat monoclonal IgM antibody against human, mouse, rat SSEA-3, unconjugated.

Supplier: STEMCELL Technologies

Serum-free culture kit for expansion of human B cells.

Supplier: STEMCELL Technologies

Serum-free medium for efficient and reproducible generation of lung progenitor cells from human ES and iPS cells.

Supplier: STEMCELL Technologies

Immunomagnetic positive selection of particle-free CD45+ leukocytes from primary human tissues and tumors.

Supplier: STEMCELL Technologies

Supplement for the differentiation of mouse naïve CD4+ T cells into Th1 cells.

Supplier: STEMCELL Technologies

Differentiation kit for the generation of astrocyte precursors from human ES and iPS cell-derived neural progenitor cells.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human, mouse CD105 (endoglin).

Supplier: STEMCELL Technologies

Mouse monoclonal IgG2b, antibody against human, chimpanzee, CD45RA, PE-conjugated.

Supplier: STEMCELL Technologies

Culture medium to initiate organoids from primary liver tissue.

Supplier: STEMCELL Technologies

The EBV (BZLF1) peptide pool is a lyophilised mixture of 59 peptides from trans-activator protein BZLF1 of Epstein-Barr virus (EBV; strain B95-8). BZLF1 is a DNA-binding protein that acts as a switch from latent infection to lytic infection. The pool consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 245 on BZLF1.

Supplier: STEMCELL Technologies

For detection and measurement of compounds that inhibit binding of viral SARS-CoV-2 Spike protein to human ACE2 receptor.

Supplier: STEMCELL Technologies

Culture medium kit for expansion of human gastric organoids.

Supplier: STEMCELL Technologies

Fluorescent dye conjugates for staining cell membranes of gram-positive bacteria, mammalian cells, and yeast bud scars - 1000 µg.

Supplier: STEMCELL Technologies

Fluorescent dye conjugates for detection of F-actin in cell culture experiments - 300 tests.

Supplier: STEMCELL Technologies

Mouse monoclonal IgG1 antibody against human angiotensin-converting enzyme 2 (ACE2).

Supplier: STEMCELL Technologies

Rat monoclonal antibody against mouse, hamster CD4, FITC-conjugated.