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Victoria blue R 80% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Victoria Blue R is a basic dye. It belongs to the class of diphenylnaphthylmethane dyes. Victoria Blue R has been employed in manufacture of lake pigments. It has lot of industrial applications, but it is rarely used as a biological stain.

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Estriol 3-sulphate sodium salt ≥98% (by TLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Estriol 3-sulfate sodium salt, (1,3,5(10)-Estratriene-3,16alpha,17beta-triol 3-sulfate; 3,16alpha,17beta-Trihydroxy-1,3,5(10)-estratriene 3-sulfate)

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Peroxidase (Myeloperoxidase) Leukocyte Kit Hanker-Yates substrate kit, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Staining system for polymorphonucleocytes in blood or bone marrow films.

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Tissue freezing medium, PolyFreeze, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Tissue samples may be snap frozen using PolyFreeze with isopentane and liquid nitrogen, dry ice (slush/slurry or bunker), or in a cryostat. PolyFreeze media do not cause autofluorescence and can easily be washed away during tissue fixation when rinsed prior to staining. Once rinsed off the slide, there is no trace of the support matrix to interfere with staining or immunohistochemistry (IHC) reactions.

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Orange G, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Orange G, Sigma-Aldrich®

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Carbol fuchsin solution, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Intended for use in the Acid Fast Stain, Procedure No. HT80 with Malachite Green counterstain Catalog No. HT80-2

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Eosin Y ∼99% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.

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Haematoxylin (Harris), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

General purpose nuclear stain, regressive type. Used with hematoxylin and eosin staining.

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Gram′s fuchsin solution for microscopy, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

May contain minor quantities of precipitated dye.

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Chromazurol B

Supplier: SIGMA ALDRICH MICROSCOPY

Chromeazurol B is a dye, belonging to the class of triphenylmethanes.

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Ponceau Xylidine ≥60 % (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Ponceau Xylidine has been used as part of the Goldner’s trichome stain to stain thin sections. It has also been used to prepare Fuchsin-Ponceau solution to stain an amyotrophic lateral sclerosis tissue-engineered

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N,N-Dimethyl-1,4-phenylenediammonium dichloride ≥99.00% (by titrimetric analysis) suitable for peroxidase analysis, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

N,N-Dimethyl-p-phenylenediamine dihydrochloride (DMPD) has been used in the HID-AB (high iron diamine-alcian blue) staining of intestinal tract samples. N,N-Dimethyl-p-phenylenediamine dihydrochloride (DMPD) is used for the determination of peroxidase. DMPD is converted into a red pigment of semiquinone nature. It is also used in the HID-AB (high iron diamine-alcian blue) staining procedures, which are used to differentiate between sialomucin and sulfomucin in the gastrointestinal tract.

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4-Nitrophthalamide 99%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

4-Nitrophthalamide,99%

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Benzophenone imine ≥95%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Benzophenone imine plays a vital role as an ammonia surrogate. It is primarily used as ammonia equivalents for the selective formation of protected primary amines. Benzophenone imine is useful as a synthetic intermediate, especially for the synthesis of glycine Schiff base. Benzophenone imine is useful for the preparation of nitrile yield dimers.

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Calcein AM 4 mM in DMSO ≥90% (by HPCE), clear solution

Supplier: SIGMA ALDRICH MICROSCOPY

Calcein AM 4 mM in DMSO ≥90% (by HPCE), clear solution

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Pararosaniline, crystalline, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Pararosaniline Base,crystalline (Parafuchsin; Paramagenta)

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Melanin 99% (by TLC), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Melanin is a ubiquitous and prominent pigment responsible for color of skin, hair and eyes. Melanin is classified into eumelanin and pheomelanin. Eumelanin is black to dark brown pigment and is present in black hair and retina of the eye. Pheomelanin is yellow to reddish brown pigment present is red hair and red feathers. Melanins are high molecular weight pigments synthesised from phenolic or indolic precursors and is widely present in animals, plants and microorganisms.

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Eosin Y 0.5 % (w/v) alcoholic solution in acidified ethanol, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.

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Dextran solution 20% (from Leuconostoc mesenteroides), autoclaved, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Use of dextrans as long and hydrophilic spacer arms to improve the performance of immobilised proteins acting on macromolecules.

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Quinoline yellow sulphonic acid sodium salt (Acid yellow 3) for microscopy, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Quinoline Yellow,for microscopy (Hist.), mixture of mono- and disulfonic acid sodium salt (Acid Yellow 3)

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Acriflavine fluorescent labeling reagent, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Acriflavine also interacts with HIF-1α (hypoxia-inducible factor 1 α) and HIF-2α, and thereby suppresses dimer formation of HIF-1 and transcriptional function. It also has negative effect on tumor growth and vascularization. Acriflavine also exhibits trypanocidal, antibacterial and antiviral activities.

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Lucifer yellow CH dilithium salt for fluorescence Stain, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

A highly fluorescent dye, useful in marking nerve cells.

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Sulpho-SMCC (3-Sulpho-N-succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate sodium salt), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

4-(N-Maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt,powder (Sulfo-SMCC)

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Zinc protoporphyrin guanylate cyclase inhibitor, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Inhibits soluble guanylate cyclase.

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Fluorinert™ FC-770, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluorinert™ belongs to the group of aliphatic compounds. It is considered as a pressure-transmitting medium because it is highly inert. Fluorinert™ Electronic Liquid FC-770 is a thermally stable, fully-fluorinated liquid that has ideal properties for use as a heat transfer fluid in a variety of industries. The inertness of Fluorinert™ FC-770 permits its use as a direct contact single and two-phase coolant in certain supercomputers and sensitive military electronics. Its high dielectric strength and electrical resistivity are ideal for applications in high voltage transformers and power electronics. In the semiconductor manufacturing industry, its wide liquid range makes Fluorinert™ liquid FC-770 suited for cooling ion implanters, dry etchers and CVD machines. Its low pour point also permits its use in thermal shock and test equipment.

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Indophenol blue 60% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Indophenol Blue is used to colourimetrically measure N-containing compounds. The free amino acids react to form a blue color complex.

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Acid Yellow 25 40% (dye content), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Acid Yellow 25, Dye content 40 %

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Tetramethylrhodamine isothiocyanate Isomer R (6-TRITC isomer R), Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Tetramethylrhodamine isothiocyanate Isomer R (TRITC) is the R isomer of tetramethylrhodamine isothiocyanate and is also known as 6-tetramethylrhodamine isothiocyanate. It is a strongly fluorescent compound soluble in ethanol, methanol, dimethylformamide, dimethylsulfoxide, and water. TRITC is a moderately-sized aminoxanthene dye carrying carboxylic acid and isothiocyanate substituents on the non-planar pendant phenyl ring. It forms a lipophilic cation in acidic pH and a hydrophilic zwitterion around neutral pH. The isothiocyanate group reacts with nucleophilic substituents (e.g., amino, hydroxyl, thiol) of biomolecules, providing the means of attaching a fluorescent label. TRITC also reacts with water and hydroxide ions and is unstable in aqueous solutions. TRITC is commercially available as a zwitterion or as a chloride.

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3,6-Dibutoxy-1,2-benzenedicarbonitrile ≥97%, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

3,6-Dibutoxy-1,2-benzenedicarbonitrile,97% (3,6-Dibutoxyphthalonitrile)

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Azure B (azure I) ≥85% (by HPLC), prepared by direct synthesis, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Azure B has been used in M′Fadyean stain to visualize Bacillus anthracis capsule. Azure B is a cationic dye. It is part of the Romanowsky stain, which is used for staining of blood films. It is also used for the localisation of nucleic acids and lignin.

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