"SIGMA ALDRICH MICROSCOPY"

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Acridine is a small cationic and planar dye that mainly binds to RNA and DNA. These dyes are strongly fluorescent. It resembles the structure of xanthene except that the heteroatom is nitrogen, not oxygen. Acridine is suitable for nucleic acid staining, particularly for cell cycle determination. Acridines are used as fluorescent materials for visualisation of biomolecules, and in laser technologies.

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Leukocyte Alkaline Phosphatase (LAP) kits are intended for the semi-quantitative demonstration of alkaline phosphatase activity in white blood cells.

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p-Rosolic acid (C.I. 43800) is an acid-baseindicator from the triphenylmethane group. It is also known as aurin and 4-[Bis(4-hydroxyphenyl)methylene]-2,5-cyclohexadienone.

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2,3,5-Triphenyltetrazolium chloride (TTC) ≥98% (by HPLC), Sigma-Aldrich®

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Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

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Chlorazol Black has been used to monitor the infection of mycorrhizal Allium porrum in plant roots. It has also been used for the staining of copepods.

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Chicago Sky Blue 6B is a counterstain for background autofluorescence in fluorescence and immunofluorescence histochemistry. It has also been used as a filling in glass pipette electrodes for electrophysiological recordings in the brain sections of rats.

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Pyrocatechol Violet (PV) is a sulfone phthalein dye. It chelates metal ions. The metal-PV complex is bluish-violet in colour.

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p-Coumaric acid has been used as a component of chemiluminescent substrate for protein detection in western blotting.

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Homovanillic acid has been used as a standard in HPLC analysis. Metabolite of dihydroxyphenylacetic acid (DOPAC) by catechol O-methyltransferase; reagent for the fluorimetric determination of glucose oxidase and other oxidative enzymes.

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Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

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Remazol Brilliant Blue R (Cl 61200) is a hydrophobic, anthraquinone dye that has several commercial uses. It is a reactive dye that stains collagen, elastin, basement membranes, and erythrocytes. Remazol Brilliant Blue R is a hydrophobic, anthraquinone dye which has several commercial uses.

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Bromobimane is also known as monobromobimane. It is also a known probe for thiols and is a fluorescent reagent activated upon a photolysis reaction.

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Popular hematology stain used for differentially staining the cellular elements of blood. For dip, rack, and batch staining techniques.

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5-Fluorouracil has been used to induce apoptosis in cells. It has been used as a chemosensitising agent. A potent antitumor agent that affects pyrimidine synthesis by inhibiting thymidylate synthetase, thus depleting intracellular dTTP pools. It is metabolized to ribonucleotides and deoxyribonucleotides, which can be incorporated into RNA and DNA. Treatment of cells with 5-FU leads to an accumulation of cells in S-phase and has been shown to induce p53 dependent apoptosis.

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Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm
bisBenzimide H 33342 trihydrochloride has been used for the staining of viable and apoptotic.