1747 Results for: "Pulverizer"

Supplier: MP Biomedicals

Methotrexate is a cell cycle arresting agent with varying effects. Methotrexate has been reported to arrest the cell cycle in late G1/S thus leading to the inhibition of the synthesis of DNA, RNA, thymidylates, and proteins. The main mechanism of action is reported to involve the inhibition of enzymes involved in purine metabolism which leads to the accumulation of adenosine or the suppression of intercellular adhesion molecule expression by T cells. Additionally, this compound has been observed to inhibit DHFR.
Methotrexate is used for chemotherapy either alone or in combination with other agents. It is effective for the treatment of a number of cancers including: breast, head and neck, leukemia, lymphoma, lung, osteosarcoma, bladder, and trophoblastic neoplasms. It is also used in treatment of autoimmune diseases, ectopic pregnancy, and for the induction of medical abortions. It is used to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites.
Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis.
Methotrexate is an allosteric inhibititor of dihydrofolate reductase (DHFR), the enzyme that catalyzes the conversion of dihydrofolate to tetrahydrofolate. Since tetrahydrolfolate is required for purine and pyrimidine synthesis, methotrexate treatment results in the inhibition of DNA and RNA synthesis.

Supplier: MP Biomedicals

Storage: +4 °C
Pyruvic acid is an intermediate in sugar metabolism and in enzymatic carbohydrate degradation (alcoholic fermentation) where it is converted to acetaldehyde and CO2 by carboxylase. In muscle, pyruvic acid (derived from glycogen) is reduced to lactic acid during exertion, which is reoxidized and partially retransformed to glycogen during rest. It improves coliform recovery when present in culture medium. It is involved in a metabolic regulatory pathway activated by mitochondrial oxidants. Pyruvate is involved in respiratory regulation in plants by interacting with alternative oxidase at a conserved cysteine residue. It may help prevent hydrogen peroxide mediated cell death.
Sodium pyruvate is utilized as a component in culture broth and media. Sodium pyruvate is used in Wallen fermentation medium to enhance the conversion of oleic acid to 10-ketostearic acid by Bacillus sphaericus. Sodium pyruvate has also been used to establish stably transfected human B cell lines.
Sodium Pyruvate has shown antioxidant properties and protective effects against oxygen radicals. Pyruvate is produced as part of glycolysis and is an intermediate in many metabolic pathways. It can be converted into acetyl CoA and enter the TCA Cycle.

Supplier: VWR Chemicals

A range of culture media in compliance with ISO standards and the International Harmonised Pharmacopoeia. All our media are, as you would expect, produced using high quality raw materials and are subject to strict quality controls, in a production environment following Good Manufacturing Practice guidelines. Raw materials are selected in compliance with the required standards. Dehydrated culture media should be stored at 10 to 30 °C unless otherwise stated, in their closed containers in a dry environment. Standard pack size is 500 g.

Supplier: GLASWARENFABRIK KARL HECHT

Clear glass wide mouth bottles, with black PP cap. Ideal for sampling, storing and transporting powders and pastes.

Supplier: MP Biomedicals

Applications:
Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Biochem/physiol Actions:
Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Typical Working Concentration:
The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C

Supplier: MP Biomedicals

Folic acid, also known as folate, is a B vitamin that can be found in a variety of fruits and vegetables. It can also be chemically synthesized. Folate, a watersoluble vitamin, helps the body form red blood cells and aids in the formation of genetic material within every body cell. This product exhibits metal binding properties. Hematopoietic vitamin present, free or combined with one or more additional molecules of L-(+)-glutamic acid, in liver, kidney, mushrooms, spinach, yeast, green leaves, and grasses.
Folic acid (Vitamin B9 and folate) is essential to numerous bodily functions. The human body needs folate to synthesize DNA, repair DNA, and methylate DNA as well as to act as a cofactor in certain biological reactions. It is especially important in aiding rapid cell division and growth, such as in infancy and pregnancy.
Folic acid (FA) and dihydrofolic acid (FAH2) are substrates of dihydrofolate reductase(s) which reduce them to tetrahydrofolate (THF), which in turn supports ‘one carbon’ transfer. Tetrahydrofolates are required for de novo synthesis of purines, thymidylic acid and various amino acids and for post-translational methylation (epigenetics).
Very slightly soluble in cold water (0.0016 mg/mL at 25 °C), soluble to about 1% in boiling water. Slightly soluble in methanol, appreciably less soluble in ethanol and butanol. Insoluble in acetone, chloroform, ether, benzene. Relatively soluble in acetic acid, phenol, pyridine, solutions of alkali hydroxides and carbonates. Soluble in hot dilute HCl and H2SO4.

Supplier: Brady

These handy boxes feature a lockable storage compartment and a group lockout compartment. Made of heavy duty steel, powder coated for extra rust resistance.

Supplier: MP Biomedicals

Agarose is a natural polysaccharide isolated from agar or agar-bearing marine algae. Its physical and chemical properties are particularly suited for use as a gel medium for diffusion and electrokinetic movement of biomolecules. Agarose is essentially biologically inert and forms relatively clear, transparent gels. Agarose based gels offer several advantages: High gel strength permits use of low concentrations. The macroporous nature of the gels allows rapid diffusion of high molecular weight molecules. Agarose is Non-toxic. Agarose gels are thermally reversible facilitating sample recovery. Staining and destaining may be done rapidly with minimal background. Because agarose forms macroporous gels that are electrically nonionic, it is an excellent medium for Isoelectric Focusing (IEF). A selection of different formulated agaroses with specific qualities for unique applications are available from MP.
Multipurpose Agarose is a versatile high gel strength agarose, especially designed for a wide range of molecular biology techniques. It is used for resolution of fragments over a large size range from 200 bp up to 50 kb by conventional electrophoresis. It is suitable for use in pulsed field gel electrophoresis and for blotting assays.
High gel strength agarose, especially designed for a wide range DNA. Versatile – separate fragments from 200 bp up to 50 kb by conventional electrophoresis. Concentrations between 0.4% and 6%. Multipurpose Agarose features the highest gel strength available, allowing 0.4 - 0.5% agarose gels. Significantly decreases electrophoresis times. Improves separation of very large fragments. Dissolves easily in a microwave.

Supplier: MP Biomedicals

Ethylenediamine tetraacetic acid is a chelator of divalent cations. It also inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Supplier: MP Biomedicals

Storage: -20°C, desiccate
This is an ultrapure NAD, chromatographically purified to remove trace inhibitors.
β-NAD, a pyridine nucleotide and biologically active form of nicotinic acid, is a coenzyme necessary for the catalytic reaction of certain enzymes. It occurs in living cells primarily in the oxidized state. Serves as a coenzyme of the dehydrogenases, especially in the dehydrogenation of primary and secondary alcohols. NAD usually acts as a hydrogen acceptor, forming NADH which then serves as a hydrogen donor in the respiratory chain.
Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NAD as a catalyst intermediate and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. The reduced form, β-NADH, is fluorescent whereas β-NAD is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M.
Electron acceptor. β-NAD is a carrier for hydride ion, forming b-NADH. Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase. Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH. Unlike b-NAD which has no absorbance at 340 nm, b-NADH absorbs at 340 nm (EmM = 6.22). The increase in absorbance at 340 nm with the formation of b-NADH is the basis for measurement of activity of many enzymes.

Supplier: MP Biomedicals

Applications
Guanidine hydrochloride can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Also used in the isolation of RNA. It is a strong chaotropic agent useful for the denaturation and subsequent refolding of protein, it can solubilize insoluble or denatured proteins such as inclusion bodies and be used for the recovery of periplasmic proteins. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary. Also used in the isolation of RNA.
Product Description
The crystal structure of Guanidine hydrochloride consists of a network of guanidinium cations and chloride anions linked by N–H•••Cl hydrogen bonds,it is a strong chaotropic agent.Guanidine HCl may agglomerate upon storage. It may appear as a free-flowing crystalline powder, a freeflowing powder with solid material dispersed throughout, or a solid. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine HCl appear identical

Grade: Ultra Pure
Purity: >99%
Keywords: Guanidium chloride, chaotropic agent
Key Applications: Chaeotropic agent
Product Type: Biochemicals
Biochemical Category: Chaotropic Agents
Density: 1.345 g/cm³ at 20 °C (Lit.)
Melting Point: 180-190 °C
UV/Visible Absorbance: OD260nm (6.0 M aq soln) <0.03
Presentation: White Crystalline Powder
pH: 4-6 (6.0 M aq soln)
Solubility: Soluble in water,Clear, Colorless Solution (6 M - clear, colorless solution).
Storage & Handling: Room Temperature, desiccate

Supplier: RSG

Stainless steel 18/10. Multipurpose spoon for a wide range of uses.

Supplier: MP Biomedicals

L-arginine is an amino acid that is necessary for the production of protein, also helps rid the body of ammonia (a waste product) and stimulates the release of insulin. In addition, L-arginine is used to make nitric oxide (a compound that relaxes the blood vessels).

Supplier: MP Biomedicals

Storage: Store at Room Temperature (15-30 °C).
Ethylenediamine Tetraacetic Acid is a polyamino carboxylic acid hexadentate ligand and a chelating agent. This product is designated as Molecular Biology grade and is suitable for molecular biology applications.
Ethylenediamine Tetraacetic Acid is a chelator of divalent cations. It also inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Supplier: EDVOTEK

Evidence left behind at a crime scene can identify a potential culprit. Even in this age of DNA, fingerprints and blood stains are still important in helping to identify a criminal. In this experiment, students solve a crime by dusting for fingerprints and use fluorescent dust to search for and identify trace amounts of blood.

Supplier: RSG

Stainless steel 18/10 blade with black plastic handle.

Supplier: Dr. Weigert

neodisher® LaboClean A8.

Supplier: Merck

BORON POWDER LAB 1 * 1 kg

Supplier: Merck

COBALT POWDER 99+ 1 * 25 g

Supplier: MP Biomedicals

Colorless, clear solution at 10 g + 100 ml, in water (Lit.).

Supplier: MP Biomedicals

Potassium sodium tartrate tetrahydrate has been used in organic synthesis to break up emulsions in aqueous workups.

Supplier: Dr. Weigert

Mildly alkaline detergent for washing machines with applications including cleaning of surgical instruments, anaesthesia materials, operating theatre clogs and glassware used in medical, biological and chemical laboratories.

Supplier: Dr. Weigert

neodisher® LaboClean GK is especially suitable for the automated cleaning of glassware for cell and tissue cultures, in the field of microbiology as well as nuclear medicine and isotope laboratories.

Supplier: BOCHEM

Stainless steel 18/10, anti-magnetic. With round handle, one flat and one scoop shaped, rounded spatula end.

Supplier: PanReac AppliChem

YT-Medium - Powder (2X) 1 * 2,5 kg

Supplier: Liebherr

HANDLE POWDERED WHITE 1 * 1 items

Supplier: BURKLE

LANCE FOR POWERPROOF SAMPLER 120CM 1 * 1 items

Supplier: MP Biomedicals

L-Histidine has been used to study cultures of the human T-lymphoblastic leukemia cell line MOLT-4 to study modulation of apoptosis. Exogenous histidine has been shown to enhance the biosynthesis of lovastatin by cultured Aspergillus terreus. Histidine has been utilised as a single nitrogen source to probe swarming in Pseudomonas aeruginosa on agar. An in vivo study has used L-histidine to diminish the net secretory response of the small intestine of of cholera toxin-challenged mice.

Supplier: Liebherr

DOOR OPENER SPARE PART 1 * 1 items

Supplier: Dr. Weigert

neodisher® LaboClean UW is suitable for the automated cleaning of laboratory glassware and utensils in water analysis laboratories, in serological institutes and in the phosphates industry. Especially for laboratory glassware used for sensitive analyses, where residues of phosphorous compounds would adulterate analysis results.