289936 Results for: "Protein+Extraction+Kits"

Supplier: Thermo Fisher Scientific

TMT10plex™ Isobaric Mass Tag Labelling Kit and Reagent Sets enable multiplex relative quantitation by mass spectrometry (MS).

Supplier: Thermo Fisher Scientific

Imject® Maleimide-activated mcKLH and PEGylated mcKLH enable conjugation of sulfhydryl-containing peptide haptens to elicit an immune response and antibody production against the hapten.

Supplier: Restek

QuEChERS methods are fast, easy, and cost-effective, and Restek Q-sep® products make QuEChERS procedures even simpler.

Supplier: Thermo Fisher Scientific

Neuronal Protein Extraction Reagent (N-PER), sufficient reagent to extract protein from ~10 g of cells or tissue, Pierce

Supplier: G-Biosciences

InGel™ Blue provides a complete set of reagents for the in-gel tryptic digestion and extraction of peptides for mass spectrometry (MALDI and LC MS/MS). The kit is specifically designed for use with Coomassie® or fluorescent stained protein spots/bands.

Supplier: Thermo Fisher Scientific

Detoxi-Gel™ endotoxin removing columns and gel uses polymixin B ligand immobilised on beaded affinity resin to bind and extract endotoxins from antibody or protein samples.

Supplier: Merck Millipore (Calbiochem‎)

Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyses amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease: protein by weight) for sequence analysis.

Supplier: G-Biosciences

The use of peptides for the generation of antibodies against specific peptides has become an essential tool in proteomic research. A crucial step in using peptides as antigens is their coupling to carrier proteins. Peptides are designed to be good epitopes by themselves; however they fail to elicit a strong antibody response. The reason for this is that the epitopes are able to bind to the B-cells, but are too small to contain additional motifs required to associate with class II molecules and T-cell receptors. The carrier proteins, once coupled to the peptides, provide these additional motifs and generate a strong antibody response. All three carrier proteins are also offered as activated carrier proteins, named as ActiveHOOK™.

Supplier: G-Biosciences

Fraction-FOCUS™ utilises proven technology to fractionate and concentrate all proteomes into multiple fractions; simplifying 2D maps and enhancing detection of low abundant proteins. Fraction-FOCUS™ is fully compatible with all downstream protein identification techniques.

Supplier: G-Biosciences

Immobilised Protein A and G are designed for binding the constant domains of immunoglobulin (Ig) molecules. Immobilised Protein A is coupled to 6% highly cross-linked agarose beads by a proprietary coupling method that provides high coupling efficiency for immunoglobulins and minimal protein A leaching. The binding capacity of Protein A is 18 to 43 mg Human IgG per ml resin.

Supplier: G-Biosciences

InGel™ provides a reliable method for the proteolytic digestion and subsequent extration of silver stained proteins in gel for subsequent analysis by mass spectrometry.

Supplier: Merck Millipore (Novagen)

BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

Supplier: Whatman products (Cytiva)

Grade 603 standard cellulose extraction thimbles for DIONEX ASE.

Supplier: Thermo Fisher Scientific

SDS-Out™ SDS Precipitation Kit provides convenient removal of SDS from small-volume (0,1 to 10 ml) protein samples.

Supplier: TCI

Capsanthin (=paprika extract) in vegetable oil solution

Supplier: TCI

Crocin (gardenia fruits extract)

Supplier: Merck Millipore (Novagen)

BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.

Supplier: Thermo Fisher Scientific

Pierce™ TMTzero™ Label Reagent serves as a "light" tag for MS-level peptide quantitation similar to duplex isotopic metabolic labeling, such as SILAC, or isotopic dimethylation labeling.

Supplier: G-Biosciences

Detergents are essential for protein solubility during protein extraction and sample preparation, especially when working with hydrophobic proteins. The presence of high concentrations of detergents in protein samples can impair ELISA, IEF, protease digestion of proteins and suppress peptide ionisation when analysed by mass spectrometry.

Supplier: Merck Millipore (Novagen)

BugBuster® Protein Extraction reagent is formulated for the gentle disruption of the cell wall of E. coli, liberating soluble protein. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The formulation utilises a detergent mix that is capable of cell wall perforation without denaturing soluble protein.

Supplier: Merck Millipore (Novagen)

BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.

Supplier: Whatman products (Cytiva)

Grade 603 standard cellulose extraction thimbles, made of high quality cellulose, are available in a range of sizes suitable for use in thimble holders and automated extraction apparatus to ensure optimal fit.

Supplier: Thermo Fisher Scientific

Tissue Protein Extraction Reagent (T-PER ) enables mild extraction of total protein from tissue samples.

Supplier: VWR Collection

Pure cellulose cotton. Suitable for all types of extractors (Soxhlet, Büchi, Gerhardt and more).

Supplier: G-Biosciences

Immobilised biotin resin is designed for the high affinity chromatography purifications of avidin, streptavidin and neutravidin protein. The resin consists of biotin coupled to 6% cross-linked agarose.

Supplier: Thermo Fisher Scientific

This extraction reagent is formulated to effectively lyse yeast and bacterial cells to extract and solubilise proteins for analysis.

Supplier: Lenz Laborglas GmbH & CO.KG

DURAN®, Borosilicate glass.

Supplier: Thermo Fisher Scientific

The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

Supplier: G-Biosciences

DetergentOUT™ Tween® is a spin column format detergent removal resin for polysorbate or Tween® detergents or surfactants.

Supplier: Thermo Fisher Scientific

The HiPPR™ Detergent Removal Spin Column Kit improves mass spectrometry results by efficiently removing detergents from 25 to 200 µl samples with low protein or peptide concentrations.