Supplier: MP Biomedicals

Soluble in 1:1 CHCl₃:methanol (50 mg/ml), soluble in DMF ((25 mg/ml).

Supplier: MP Biomedicals

Collagenase is a protease which cleaves the triple-helical protein called collagen. It has extensive use in biological studies, where it is used to prepare isolated cell suspensions.

Supplier: MP Biomedicals

Roseoflavin is a weak antibacterial agent active against some gram-positive bacteria, especially Staphylococcus aureus, Sarcina latea, Bacillus cereus and Bacillus subtilis. Also exhibits antiriboflavin activity and inhibits the biosynthesis of riboflavin by a repressor mechanism.

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Ionomycin is an ionophore produced by the bacterium Streptomyces conglobatus.A polyether ionic antibiotic that exhibits an affinity for Ca²⁺. It is used in research to raise the intracellular level of calcium (Ca²⁺) and as a research tool to understand Ca²⁺ transport across biological membranes. These cytokines are important in the inflammatory response.

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Most cells can synthesise non-essential amino acids (NEAA) through glucoaminolysis, glycolysis, or the TCA cycle. When these amino acids concentration levels are low, cells need to consume more glucose and glutamine in the media and produce excess by-products, which might affect cell growth. Adding NEAA into the cell culture medium could mitigate the potential side effects caused by self-production of these non-essential amino acids.

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Mono-Poly® Resolving Medium is used to separate mononuclear and polymorphonuclear leukocytes from whole blood in two distinct bands. Mono-Poly® is highly versatile and can be used for a variety of research and clinical applications, such as histocompatibility testing, lymphocyte studies, in vitro cell mediated immunity assays, or any procedure requiring pure white blood cell preparation.

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Lymphocyte separation medium, LYMPHOSEP® is a sterile, iso-osmotic, low viscosity solution for density centrifugation yielding a clear separation of lymphocytes from heparinised or defibrinated human blood.

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RPMI 1640 medium was originally developed to culture human leukaemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented.

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Double antibiotic solution containing 5,000 units/mL of penicillin and 5,000 µg/mL of streptomycin.

Supplier: MP Biomedicals

Storage: Room Temperature, desiccate.
Acrylamide is prepared on an industrial scale by the hydrolysis of acrylonitrile by nitrile hydratase.
Acrylamide is a monomer used in a variety of synthetic processes to form polymers and copolymers. It polymerizes in the presence of free radicals in aqueous solutions. It is usually used to prepare polyacrylamide gels for electrophoresis separation of biomolecules.
Acrylamide is an extremely hazardous substance in either the powder form or liquid form. Once polymerized, the polyacrylamide is considered safe to handle under normal laboratory conditions. Acrylic Acid Content -≤0.001%

Supplier: MP Biomedicals

Storage: Store at +4 °C, store under nitrogen
Glutathione is the major low molecular weight thiol compound of the living plant or animal cell. It is a tripeptide with a gamma peptide linkage between the amine group of cysteine (which is attached by normal peptide linkage to a glycine) and the carboxyl group of the glutamate side-chain. It is an antioxidant, preventing damage to important cellular components caused by reactive oxygen species such as free radicals and peroxides. The sulfhydryl (thiol) group (SH) of cysteine serves as a proton donor and is responsible for the biological activity of glutathione.
Glutathione suppresses human immunodeficiency virus expression in chronically infected monocytic cells. It is a useful tripeptide involved in many aspects of metabolism, including transport of g-glutanyl amino acids and reductive cleavage of disulfide bonds.
Endogenous antioxidant that plays a major role in reducing reactive oxygen species formed during cellular metabolism and the respiratory burst. Glutathione-S-transferase catalyzes the formation of glutathione thioethers with xenobiotics, leukotrienes, and other molecules that have an electrophilic center. Glutathione also forms disulfide bonds with cysteine residues in proteins. Via these mechanisms, it can have the paradoxical effect of reducing the efficacy of anti-cancer agents.

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This mouse chondroitin 6 sulphate monoclonal antibody used in EIA, RIA and immunoblotting.

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Freund's complete adjuvant is made by a modification of Freund's technique, in which 25 mg of Mycobacterium is suspended in a mixture of 7,5 ml Arlacel A and 42,5 ml paraffin oil.

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The Ouchterlony Plates have eight antigen wells (8 mm diameter), each surrounded by six antisera wells (3 mm diameter). These plates have been especially designed for work involving antigens present in very low concentrations. The large antigen well allows 75 μl of sample to be transferred at one time. The format is particularly useful for isotyping of tissue culture supernatants of monoclonal antibodies.

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Used to minimise cell attachment or aggregation.

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MPure-12™ is a bench top automated system for rapid purification of nucleic acids from a wide variety of bio-specimens using magnetic bead separation technology. It has a uniquely designed magnetic bead processing chamber and is fully integrated with easy to use pre-packaged reagent kits.

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Ready to use 5% cleaning solution.

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Pyrazinamide is used therapeutically as an antitubercular agent.

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Glass beads.

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The FastDNA™ SPIN Kit for Soil is designed to efficiently isolate bacterial, fungi, plant and animal genomic DNA from soil and environmental samples.

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Streptozotocin, a glucosamine-nitrosourea, is a DNA alkylating agent that enters cells exclusively via the GLUT2 glucose transport protein. Streptozocin, a diabetagen, is especially toxic to pancreatic islet insulin-producing β-cells. It is toxic to GLUT2 positive neuroendocrine tumor cells.

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Puromycin is nucleoside antibiotic & protein synthesis inhibitor that causes premature chain termination by acting as an analogue of the 3′-terminal end of aminoacyl-tRNA. Prevents growth of bacteria, protozoa, algae, and mammalian cells.

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Minimum Essential Medium Eagle With Earle's Salts and 2,0 g/L sodium bicarbonate Without L-glutamine, L-cysteine, L-cystine, and L-methionine is generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera.

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BetaMax™ ES, as its name implies, is the most efficient environmentally safe LSC cocktail for beta particle counting in non-aqueous samples.

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Acetyl-CoA is produced via beta-oxidation of fatty acids, via the metabolism of carbohydrates - glucose 6-phosphate to pyruvate to acetyl-CoA and via the catabolism of amino acids. Acetyl-CoA has a number of metabolic opportunities. It is metabolised in the tricarboxylic acid cycle to produce carbon dioxide, water and energy.

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Tobramycin is an aminoglycoside antibiotic derived from Streptomyces tenebrarius and used to treat various types of bacteria infections, particularly Gram-negative infections. It is especially effective against species of Pseudomonas. It works by binding to a site on bacterial 30S and 50S ribosome, preventing formation of the 70S complex. As a result, mRNA cannot be translated into protein and cell death ensues. Tobramycin is preferred over gentamicin for Pseudomonas aeruginosa pneumonia due to better lung penetration.

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Soluble in chloroform (50 mg/ml - clear colorless solution).

Supplier: MP Biomedicals

Suspend 30 g of the medium in one liter of distilled or deionised water. Mix well. Heat slowly until the medium is dissolved. Dispense and sterilise at 121 °C (15 lbs of pressure) for 15 minutes.

Supplier: MP Biomedicals

Ammonium Molybdate Tetrahydrate is an analgesic.

Supplier: MP Biomedicals

There is approximately one HBr or HCl per lysine unit. The HBr/HCl allows it to be a crystalline solid and soluble in water. To remove the HBr/HCl, solubilise the product in a neutral buffer and dialyse away the salts.