SpinPrep™ PCR clean up kit
Catalog # 70976-3
Supplier: Merck Millipore (Novagen)
CAS Number:
Specifications
- Pk:100 Tests
- Method / Format:Column (silica-based)
- Prep size:Mini
- Refine target molecule:Amplified/modified DNA
- Sample type:PCR and other enzymatic reactions
- Target molecule:DNA
- Description:SpinPrep™ PCR® Clean-Up Kit
- Description:SpinPrep™ PCR® Clean-Up Kit
Specifications
About this item
The SpinPrep™ PCR Clean Up Kit is designed for the rapid purification of DNA amplified by PCR. The 10-minute procedure involves addition of a binding buffer followed by adsorption of the DNA to a silica membrane in a spin column format. Following a wash step, the DNA is eluted in low salt buffer. This kit removes DNA polymerises, dNTPs, salts, and >99% of primers so that they do not interfere with downstream applications such as cloning, sequencing, or labelling. PCR products from 100 to >12000 bp can be cleaned up, with recoveries of amplified DNA in the range of 60 to 90% under standard conditions.