About this item
IL-4 is an immunomodulatory cytokine that stimulates B cell proliferation and activates eosinophils, basophils, and mast cells.
- High quality
- Low endotoxin
- Lot-to-lot consistency
- Animal free
- Manufactured following GMP guidelines
Induces vascular cell adhesion molecule (VCAM)-1 expression on endothelial cells and involved in collagen production by fibroblasts
Plays a key role in promotion of Th2 differentiation from naive helper T cells
Defines the Th2 phenotype of lymphocytes
Regulates cell proliferation, apoptosis, and expression of numerous genes in various cell types
The biological activity of CTGrade GMP interleukins and growth factors is standardised, where applicable, to WHO International standards, providing cell and gene therapy developers consistent, lot-to-lot biological activity and performance
Host Cell Protein: ≤30 ng/ml
Host Cell DNA: ≤0,01 ng/ml
Sterility testing: No Growth
Mycoplasma: Negative
Specifications
- Conjugation:Unconjugated
- Protein function:Cytokine
- Protein/peptide type:Recombinant
- Source:E. coli
- Species:Human
- Storage conditions:At or below –20 °C
- Endotoxin content:Endotoxin LAL (EU/µg) ≤0,05
- Biological activity:Bioactivity Assay: TF-1 cell proliferation, ED₅₀ ≤0,25 ng/ml, Bioactivity ≥1,4x10⁴ IU/µg
- Gene ID:P05112
- Reconstitution instructions:Sterile water at 0,1 - 0,5 mg/ml
- Endotoxin-free:N
- Carrier-free:Y
- Protease-free:N
- Animal-free:Y
- Protein synonyms:BCGF|B-cell stimulatory factor-1|BCDF|BSF-1
- Protein/peptide name:CTGrade GMP IL-4
- Purity:≥98%
- Molecular weight:15,1 kDa
- Sequence:MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS
- Endotoxin level:Low
- Formulation:20 mM Sodium phosphate, pH 7,5
- Nuclease-free:N
- Grade:GMP
- Shipping temperature:Ambient
- Tested applications:Bioassay