About this item
A centrifugal tool for purifying and concentrating His-tagged proteins, GSH-tagged proteins and antibodies.
- Minimum final concentrate volume: 15 µl
- Colour code: Clear with pink cap
- Non sterile
- Ø×L: 297×120 mm
The device combines affinity-based spin column purification with downstream sample concentration and buffer exchange. Featuring a large reservoir that accommodates a range of sample volumes, the device reduces the need for multiple centrifugation steps. Simply attach the included Amicon® Ultra filter for simultaneous elution, concentration and highly efficient buffer exchange (>99%) in a single spin.
Material:
Amicon Ultra styrene-butadiene device
Ultracel® regenerated cellulose membrane (Amicon Ultra-0.5; low binding)
Polyethylene cap
Polyethylene frit
Polypropylene device (for exchange)
Polypropylene frit
Polypropylene tube (AU0.5; for collection)
Polypropylene tube (for collection)
Polypropylene tube (holder)
Applications: Affinity purification, depletion of abundant proteins, protein enrichment, buffer exchange, desalting, clean-up of antibody labelling reactions
Kit includes 12/pk Amicon Pro, 12/pk Amicon Ultra NMWCO 3/10/30/50/100 kDa, 3 ml HisBind Ni-NTA resin (Binding capacity: 5 to 10 mg His tagged protein/ml agarose), 25 ml bind buffer (4X), 25 ml wash buffer (4X), 5 ml elution buffer (4X).
Amicon® Pro affinity concentration kit Protein A:
Kit includes 12/pk Amicon Pro, 12/pk Amicon Ultra NMWCO 3/10/30/50/100 kDa, 3 ml Protein A resin (Binding capacity: 20 mg human IgG/ml agarose), 2×66 ml bind/wash buffer, 12 ml elution buffer, 2,5 ml neutralisation buffer.
Amicon® Pro affinity concentration kit Protein G:
Kit includes 12/pk Amicon Pro, 12/pk Amicon Ultra NMWCO 3/10/30/50/100 kDa, 3 ml Protein G resin (Binding capacity: 20 mg human IgG/ml agarose), 2×66 ml bind/wash buffer, 12 ml elution buffer, 2,5 ml neutralisation buffer.
Amicon® Pro affinity concentration kit GST:
Kit includes 12/pk Amicon Pro, 12/pk Amicon Ultra NMWCO 3/10/30/50/100 kDa, 3 ml GST bind resin (Binding capacity: 5 to 8 mg GST tagged protein/ml agarose), 125 mg glutathione (reduced, free acid), 25 ml GST bind/wash buffer (10X), 5 ml GST reconstitution buffer (10X)
Related Information
Merck Protein Buffer Optimization and Sample Concentration.pdf