CD14⁺ T cells, obtained from mononuclear cells, are isolated through either negative or positive selection using immunogenetic cell separation techniques. These cells play a crucial role in the immune response against infections and are valuable for the investigation and diagnosis of immune reactions, particularly in infections and inflammation.

• Their ability to act as detectors for bacterial presence and respond to lipopolysaccharides (LPS) underscores their significance in combating infections through phagocytosis, as well as in maintaining immune balance and defending against microbial threats.

Cells were collected from from a healthy human donors using permission forms and procedures that have been approved by the Institutional Review Board (IRB). Storage: For prolonged cell preservation or resuscitation, it is recommended to prepare the cells for extended storage in the vapor phase of liquid nitrogen. Storage of cells in the liquid phase of nitrogen is strongly discouraged to prevent potential cross-contamination risks when removing from storage. Long-term storage under −175 °C and for short-term storage, about one or two weeks at −80 °C is permitted, but it is recommended to minimise this duration to ensure optimal stability. Thawed samples should be used immediately and not stored.