CytoLiner™ Fixed cell membrane dyes are novel lipophilic fluorescent dyes developed specifically for selective plasma membrane staining in fixed cells for microscopy. The dyes are engineered for robust and consistent staining of formaldehyde-fixed cells, and are suitable for downstream immunofluorescence staining protocols.

• Robust and consistent staining of plasma membrane in formaldehyde-fixed cells

• Suitable for antibody co-staining
• Compatible with poly-L-lysine coated cultureware
• Available in 5 colours from blue to near-IR

While classic lipophilic membrane dyes like DiI can be used to stain formaldehyde-fixed cells, staining can be highly variable due to the poor solubility of the dyes. CytoLiner™ Dyes are a new generation of membrane dyes uniquely engineered to permit selective staining of the plasma membrane in fixed and mildly permeabilized cells.

CytoLiner™ staining is compatible with formaldehyde fixation and mild detergent permeabilization before staining. Staining can tolerate blocking agents used during immunofluorescence staining protocols, allowing subsequent staining with antibodies and other probes. The dyes also are compatible with poly-L-lysine coated cultureware and Transwell® permeable filter supports. CytoLiner™ Dyes are available in a selection of 5 colors from blue to near-IR.

Fix before staining (formaldehyde), permeabilize before staining.