The rLSL-N is an Escherichia coli recombinant of N-terminal domain of carbohydrate-binding protein (Lectin) derived from Laetiporus sulphureus. This lectin preferably binds to [Galβ(1-4)GlcNAc, poly LacNAc] glycan epitopes (It very slightly interacts with lactose).
The LecBeads is a lectin-immobilized agarose which is capable of capturing glycoproteins via binding to its specific epitope(s), and it is prepared by a covalent coupling method between lectin and agarose.
The Lectin-catch method using LecBeads (as shown in Application tab) is available for selective separation of glycoconjugates (glycoproteins, glycolipids, and so on). This LSL-N-LecBeads (1 ml gel) shows a binding capacity of about 12 mg or more asialo human Transferrin [asialo hTF] through its binding to a terminal [Galβ(1-4)GlcNAc] glycan epitope on asialo hTF.

• Preparation : Recombinant LSL-N is covalently coupled with agarose gel
• Quantity : 1 ml gel/2 ml (1:1, v/v slurry) suspended in PBS, pH7.5
• Protein concentration : about 10 mg/ml gel
• Binding capacity : >12 mg of glycoprotein (asialo human Transferrin) /ml gel
• Preservative : 0.02% [w/v] Sodium azide
• Stabilizer : None
• Store at 2-10 °C. (Avoid freezing).