ArciTect™ tracrRNA Kit

Supplier: STEMCELL Technologies

Certificates

Supplier: STEMCELL Technologies

About this item

Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing.

ArciTect™ tracrRNA is a trans-activating CRISPR RNA (crRNA), one of two RNA components required to make a guide RNA (gRNA) for CRISPR-Cas9 genome editing. ArciTect™ tracrRNA contains a complementary sequence to the linker sequence in ArciTect™ crRNA, allowing fast and efficient gRNA duplex formation. ArciTect™ tracrRNA is recognized by all ArciTect™ Cas9 nucleases and is essential for ribonucleoprotein (RNP) complex formation. It contains 2'-O-methyl and phosphorothioate modifications at the first two 5' and 3' terminal residues for optimal stability and editing efficiency. The ArciTect™ tracrRNA Kit includes tracrRNA as well as ArciTect™ Annealing Buffer (5X), which is required for forming a gRNA duplex between ArciTect™ tracrRNA and ArciTect™ crRNA. ArciTect™ crRNA is available by custom order for user-specified target sequences, in 2, 10 and 20 nmol sizes. ArciTect™ Cas9 Nuclease is also available separately .

Caution: Product restrictions: (i) Customer shall not make products or any portion of them in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier and (ii) Customer is familiar with Supplier’s restrictions on the use of the products and shall use the products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').

Specifications

Cell specific:
Pluripotent stem cells