Animal component-free medium and substrate for derivation and expansion of human mesenchymal stromal cells from umbilical cord tissue.

MesenCult™-ACF Plus Umbilical Cord Culture Kit includes standardized, animal component- and serum-free media for the derivation and expansion of human mesenchymal stromal cells (also known as mesenchymal stem cells; MSCs) from umbilical cord tissue. This kit works together with complete MesenCult™-ACF Plus Medium for the long-term expansion of umbilical cord-derived MSCs. MesenCult™-ACF Plus Umbilical Cord Medium must be used in conjunction with Animal Component-Free Cell Attachment Substrate and Animal Component-Free Cell Dissociation Kit (Catalog #05426) to provide a complete animal component-free (ACF) culture system. Components of Animal Component-Free Cell Attachment Substrate and Animal Component-Free Cell Dissociation Kit are pre-screened and tested for optimal cell adherence when cells are cultured with MesenCult™-ACF Plus Umbilical Cord Culture Kit and MesenCult™-ACF Plus Medium. For optimal animal component-free cryopreservation, MesenCult™-ACF Freezing Medium (Catalog #05490) is recommended for human MSCs previously cultured in MesenCult™ media.

NOTE: L-Glutamine (Catalog #07100) is required for preparation of MesenCult™-ACF Plus Umbilical Cord Medium and complete MesenCult™-ACF Plus Medium; see Preparation of Reagents and Materials. CollPlant is the manufacturer of the rhCollagen component of the cell attachment substrate. The Product may be used for research purposes only. Please contact STEMCELL for any clinical or commercial application of the Product.