Culture medium kit for establishment and maturation of human cerebral organoids.

Generate self-organized, pluripotent stem cell (PSC)-derived neural organoids with a cellular composition and structural organization representative of the developing human brain. These defined, serum-free cell culture media and the simple, four-stage protocol are based on the formulation published by Lancaster et al. to more reliably generate cerebral organoids.

Beginning with an embryoid body (EB) formation step followed by expansion of neuroepithelia, organoids generated using STEMdiff™ Cerebral Organoid Kit feature cortical-like regions, including the ventricular zone (PAX6+/SOX2+/Ki-67+), outer subventricular zone (Ki-67+/p-Vimentin+), intermediate zone (TBR2+), and cortical plate (CTIP2+/MAP2+/TBR1+), which layer in similar orientations as those observed in vivo. For extended culture periods (> 40 days), the components required for maturation can be purchased as STEMdiff™ Cerebral Organoid Maturation Kit.