Cell culture medium kit for robust generation of dorsal forebrain neural organoids from human pluripotent stem cells.

Robustly generate three-dimensional, patterned brain organoid cultures from human pluripotent stem cells without matrix embedding. STEMdiff™ Dorsal and Ventral Forebrain Organoid Differentiation Kits are serum-free cell culture media that work with AggreWell™-generated embryoid bodies to prevent organoid fusion and enable the scalable generation of over 500 highly reproducible organoids per kit. Adapted from protocols, these brain-region-specific organoids are three-dimensional in vitro models with a cellular composition and structural organisation that is representative of the developing human forebrain.

STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit generates tissue of the early developing dorsal pallium, while STEMdiff™ Ventral Forebrain Organoid Differentiation Kit generates tissue of the early developing ventral subpallium. Organoids generated with these kits can also be co-cultured as assembloids to study brain region interactions. For extended periods of organoid culture (>50 days), the components required for organoid maintenance are available as STEMdiff™ Neural Organoid Maintenance Kit.