Serum-free culture medium kit for differentiation of human PSCs to atrial cardiomyocytes.

STEMdiff™ atrial cardiomyocyte differentiation kit is optimised for the differentiation and maintenance of human embryonic stem and induced pluripotent stem cells into atrial cardiomyocytes (cardiac troponin T-positive; cTnT+). This serum-free kit can be used to generate atrial cardiomyocytes derived from a clump culture of human pluripotent stem cells (hPSCs) maintained in mTeSR™1, mTeSR™ plus, or TeSR™-E8™. With greater than 80% yield purity, an average of 1×10⁶ cTnT+ cells can be harvested from a single well of a 12-well plate. STEMdiff™ cardiomyocyte maintenance kit comprises the maintenance basal medium and supplement and allows for long-term maintenance of hPSC-derived cardiomyocytes for one month or longer. These cardiomyocytes can be used in various downstream applications and analyses.