STEMdiff™ SMADi Neural Induction Kits

Serum-free medium kit for highly efficient SMAD inhibition-mediated neural induction of human ES and iPS cells.

STEMdiff™ SMADi neural induction kit consists of a defined, serum-free medium and supplement for the highly efficient neural induction of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. This kit combines STEMdiff™ neural induction medium with STEMdiff™ SMADi neural induction supplement, which directs differentiation by blocking TGF-β/BMP-dependent SMAD signaling, resulting in efficient neural induction of even hard-to-differentiate cell lines. Neural progenitor cells (NPCs) can be generated using STEMdiff™ SMADi neural induction kit with either an embryoid body (EB) protocol or monolayer culture protocol. The resulting cultures are enriched for central nervous system (CNS)-type NPCs, which express SOX1, Nestin, and PAX6. NPCs generated using this kit can be passaged as single cells and expanded in STEMdiff™ neural progenitor medium. The NPCs can also be differentiated into neurons and glia.Learn how to generate neural progenitor cells from human pluripotent stem cells (hPSCs) in our on-demand neural induction course, and browse our tech tips on the neural induction of hPSCs using the embryoid body method or monolayer method.

: Product restrictions: (i) Customer shall not make products or any portion of them in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier and (ii) Customer is familiar with Supplier’s restrictions on the use of the products and shall use the products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').