Ovary Lysate
Catalog # PRSI1316-0.1
Supplier: ProSci Inc.
CAS Number:
Specifications
- Cell Format:Cryopreserved
- Pk:0,1 mg
- Description:Normal, ovary tissues, human source
- Storage conditions:Store at 2...8 °C for continuous use. For extended storage, freeze working aliquots at −70 °C.
- Storage buffer:Ovary lysate is supplied in SDS sample buffer containing 5% β-mercaptoethanol.
- Concentration:2 mg/ml
- Tested applications:WB
Specifications
About this item
Ovary tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH 7,9, 1,5 mM MgCl₂, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors).
Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1×SDS sample buffer (50 mM Tris-HCl pH 6,8, 12,5% glycerol, 1% sodium dodecylsulfate, 0,01% bromophenol blue) containing 5% β-mercaptoethanol.