For direct quantification of cAMP from a variety of biological samples.

• A sensitive assay for cAMP in multiple sample matricies without the need for acetylation
• Assay 24 samples in triplicate or 36 samples in duplicate
• Measure cAMP levels down to 0,6 pmol/ml
• Incubation: 18 hours | Development: 90 to 120 minutes | Read: Colorimetric at 405 tp 420 nm

Cayman’s cAMP Select assay is a competitive ELISA that permits cAMP measurements within the standard curve range of 0,09 to 200 pmol/ml and a sensitivity (80% B/B₀) of approximately 0,6 pmol/ml. Acetylation of samples is not required in this highly sensitive assay where culture media, urine, tissue, and plasma samples have been validated. Cyclic AMP is a ubiquitous cellular second messenger that is a critical component of a signal transduction pathway linking membrane receptors and their ligands to the activation of internal cellular enzymatic activity and gene expression. cAMP is synthesized from ATP by membrane-bound adenylate cyclase. Binding of certain ligands or hormones to their specific G-protein couple receptors activates GTP binding proteins (G_s or G_i) which either stimulate or inhibit adenylate cyclase. cAMP activates or inhibits various enzymes or cascade of enzymes by promoting their phosphorylation or dephosphorylation. The cAMP signal is neutralized by hydrolysis of cAMP to AMP by phosphodiesterases. Therefore, the concentration of cAMP in a cell is a function of the ratio of the rate of synthesis from ATP by adenylate cyclase and its rate of breakdown to AMP by specific phosphodiesterases.