Human Recombinant TNF-A (Animal free) (from E. coli)
Supplier: Shenandoah Biotechnology
Certificates
About this item
Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS).
- High quality
- Low endotoxin
- Affordable
TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.
Animal-Free (AF) proteins are identical to regular proteins and include additional documentation showing that they were produced with no materials of animal or human origin using ANIMAL-FREE purification processes.
Delivery: A certificate of analysis (COA) is supplied with each product shipped detailing purity, formulation, biological activity, endotoxin levels, reconstitution instructions and recommended storage conditions.
Caution: For research use only.
Specifications
- Conjugation:Unconjugated
- Protein function:Cytokine
- Protein/peptide type:Recombinant
- Source:E. coli
- Species:Human
- Storage conditions:−20 °C
- Endotoxin content:Endotoxin LAL (EU/µg) ≤1
- Biological activity:Bioactivity assay: Cytolysis of mouse L929 cells in the presence of actinomycin D, ED50 ≤2 ng/ml, bioactivity ≥5,0×10⁵ units/mg
- Gene ID:P01375
- Reconstitution instructions:Sterile water at 0,1 mg/ml
- Endotoxin-free:N
- Carrier-free:Y
- Protease-free:N
- Animal-free:Y
- Protein synonyms:DIF|cachexin|cachectin|necrosin|TNFSF2|TNF|cytotoxin
- UniProtKB:P01375
- Protein/peptide name:TNF-alpha
- Purity:≥95%
- Molecular weight:17,5 kDa
- Sequence:MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
- Endotoxin level:Low
- Formulation:10 mM sodium phosphate, pH 7,5
- Nuclease-free:N
- Grade:RUO
- Shipping temperature:RT
- Tested applications:Bioassay
Specifications
Related Information
100-111AF-100UG 100-111AF-10UG
Datasheet
100-111AF-500UG
Product Literature
Literature reference - PubMed ID 24563081 Literature reference - PubMed ID 28637896