3D-FectIN™ is a 3D-transfection reagent specifically developed to directly transfect cells cultured in 3D hydrogels.

• Highly efficient for cell lines and primary cells
• Specific for 3D hydrogels
• Compatible with all types of nucleic acids
• Longterm protein expression
• Serum compatible

Suitable for all type of nucleic acids including plasmid DNA, linearised DNA, double stranded RNA, mRNA, shRNA, oligonucleotides.

3D-FectIN™/DNA complexes are colonised by cells which are subsequently transfected in situ and in a more natural environment. This method allows studying angiogenesis, tube and acini formation, colonisation, neurite growth, tissue engineering, tissue regeneration, tumour invasion, neural differentiation, cellular polarisation and tissue formation.