Sensitive chromogenic MMP substrate

Chromogenic substrate for continuous spectrophotometric assay of most matrix metalloproteinases, and TACE (ADAM17). The MMP cleavage site peptide bond is replaced by a thioester bond in this peptide. Hydrolysis of this bond by an MMP produces a sulfhydryl group, which reacts with DTNB [5,5'-dithiobis(2-nitrobenzoic acid), Ellman's Reagent] to form 2-nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm.