Neutrophils are derived from a dextran red cell sedimented population of cells. Following red cell sedimentation, granulocytes are separated using a density gradient and red cell lysis. Cells are then purified through the use of indirect selection.
- Cryopreserved in CryoStor® CS10 media
- Purity of >90% by flow cytometry
- Test negative for Hepatitis B, Hepatitis C, HIV 1/2, HTLV-I/II, HIV-1/HCV/HBV nucleic acid, WNV nucleic acid, Trypanasoma cruzi and Syphilis
Neutrophils are the most abundant type of white blood cells and form an essential part of the innate immune system. Neutrophils are one of the first-responders of inflammatory cells arriving to the site of injury within minutes of trauma. The mature neutrophil lives only a few hours or perhaps a little longer after migrating to the tissues. A large number of neutrophils are held in reserve in the bone marrow to accommodate for their short life span.
Storage: Either prepare cells for long term storage in liquid nitrogen vapor phase or thaw for use. Storage in liquid phase nitrogen is NOT recommended. Short term storage of cells (less than 2 weeks) at −80 °C is acceptable, but should be minimised to ensure maximum stability. Once thawed, samples must be used immediately.