Mouse IL1 alpha (from E. coli)

Mouse IL1 alpha (from E. coli)

Source:
E. coli
Species:
Mouse
Storage conditions:
Store at 4 °C for up to two weeks. For long term storage, aliquot and store at −20 °C, avoid freeze/thaw cycles.
Biological activity:
The ED50 as determined by the dose-dependant stimulation of murine D10S cells is 0.01 ng/ml, corresponding to Specific Activity of 100,000,000 IU/mg.
Protein synonyms:
Hematopoietin 1
Protein/peptide name:
IL1 alpha
Purity:
> 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
Solubility:
It is recommended to reconstitute the lyophilized Interleukin 1a in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Ala-Pro-Tyr-Thr.
Formulation:
The protein was lyophilized from concentrated (1mg/ml) sterile solution containing 1X PBS, pH=7.4.
Tested applications:
SDS-PAGE , HPLC , FuncS