Protein IgG Plus orientation kits, Pierce™
Pierce™ Protein IgG Plus Orientation Kits uses a crosslinker to covalently attach antibody that is bound to Protein A (or Protein G) agarose resin, thereby creating a reusable affinity column for purification of antigens.
- Contaminant free antigen purification and less antibody leaching
- Covalent, leak resistant antibody immobilisation
- Gentle binding and crosslinking conditions
- Less antibody leaching
- Crosslinked 6% beaded agarose with recombinant Protein A (or Protein G) thus making it high quality resin
- Oriented immobilisation that is able to capture passing antigen in the mobile phase without steric hindrance
: Kit includes Recombinant Protein A [or Protein G] columns, 2 ml (2 columns), disuccinimidyl suberate [DSS] (2×13 mg), PBS pack (makes 500 ml), blocking buffer (6 ml), IgG elution buffer (15 ml), antibody binding/wash buffer (120 ml), and column accessories.