The Pierce™ S-Nitrosylation Western Blot Kit enables sensitive detection of protein S-nitrosocysteine post-translational modifications.

• Optimised labeling reactions switch S-nitrosocysteine post translational modifications with iodoTMT™ reagent for easy detection by Western blot using anti-TMT™ antibody
• Uses a non-biological iodoTMT™ reagent for labeling instead of HPDP-biotin, resulting in less background during Western blot detection
• Labeling method is compatible with alternative methods of analysis, including enrichment via anti-TMT™ resin and multiplex quantitation by mass spectrometry
• All needed reagents included one kit

The Pierce™ S-Nitrosylation Western Blot Kit contains a cell lysis/reaction buffer, a sulfhydryl-reactive blocking agent, a reducing agent, a labeling agent and a detection monoclonal antibody. Unmodified cysteines are first blocked using a sulfhydryl-reactive compound (MMTS). S-nitrosylated cysteines are then selectively reduced with ascorbate in HENS Buffer for specific labeling with iodoTMTzero™ reagents, which irreversibly bind to the cysteine thiol that was S-nitrosylated. Detection of the TMT™ reagent-modified proteins is facilitated using an anti-TMT™ antibody.